Birch-Andersen A, Ferguson D J, Pontefract R D
Acta Pathol Microbiol Scand B. 1976 Aug;84(4):235-9. doi: 10.1111/j.1699-0463.1976.tb01931.x.
A double sectioning technique is described for obtaining thin sections of coccidian oocysts. This method employs cryostat sectioning of unfixed oocysts prior to treatment by normal methods of fixation, dehydration, embedding and thin sectioning for electron microscopy. A number of the oocysts treated by this technique were well preserved anc contained organelles with normal ultrastructure.
描述了一种用于获得球虫虫卵囊薄切片的双重切片技术。该方法采用在通过常规固定、脱水、包埋和超薄切片等电子显微镜处理方法之前，对未固定的卵囊进行低温恒冷切片。通过该技术处理的许多卵囊保存良好，并且含有超微结构正常的细胞器。
