通过非同位素原位杂交检测尖锐湿疣和宫颈上皮内瘤变病变中的人乳头瘤病毒感染
Detection of human papillomavirus infection by non-isotopic in situ hybridisation in condylomatous and CIN lesions.
作者信息
Pöllänen R, Vuopala S, Lehto V P
机构信息
University of Oulu, Department of Obstetrics and Gynaecology, Finland.
出版信息
J Clin Pathol. 1993 Oct;46(10):936-9. doi: 10.1136/jcp.46.10.936.
AIMS
To study the value of non-isotopic in situ hybridisation (NISH) in detecting human papillomavirus (HPV) infection in female genital lesions positive for the virus by conventional histology but negative by filter DNA hybridisation.
METHODS
Forty three cases, which showed the histological hallmarks of the HPV infection but produced negative results in filter dot blot hybridisation tests (Vira Pap and Vira Type kits), were identified in the course of an investigation of 304 vaginal, vulvar, and cervical samples from 267 patients. These cases were studied by NISH for the presence of HPV infection.
RESULTS
In 28 (65%) of the cases NISH gave a positive hybridisation signal. In 26 cases (96%) the signal was diffuse, and in two (4%) punctate or diffuse, representing episomal and episomal or integrated HPV DNA, respectively. In most cases only a few HPV positive cells were discernible.
CONCLUSION
NISH is a more sensitive technique than dot blot hybridisation, detecting HPV infection in most cases which show histological HPV atypia but which remain negative in filter DNA hybridisation. Thus NISH is useful as an additional technique to verify the presence of HPV in lesions which remain negative in filter hybridisation tests.
目的
研究非同位素原位杂交（NISH）在检测女性生殖器病变中人类乳头瘤病毒（HPV）感染的价值，这些病变经传统组织学检查呈病毒阳性，但滤膜DNA杂交检测呈阴性。
方法
在对267例患者的304份阴道、外阴和宫颈样本进行调查的过程中，发现43例病例，这些病例显示出HPV感染的组织学特征，但在滤膜斑点杂交试验（Vira Pap和Vira Type试剂盒）中结果为阴性。采用NISH对这些病例进行HPV感染检测。
结果
28例（65%）病例NISH杂交信号呈阳性。26例（96%）信号为弥漫性，2例（4%）为点状或弥漫性，分别代表游离型和游离型或整合型HPV DNA。大多数病例中仅能辨认出少数HPV阳性细胞。
结论
NISH是一种比斑点杂交更敏感的技术，能在大多数组织学显示HPV异型但滤膜DNA杂交仍为阴性的病例中检测到HPV感染。因此，NISH作为一项辅助技术，可用于验证滤膜杂交检测呈阴性的病变中HPV的存在。
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