Tomita Y, Kubota K, Kasai T, Sekiya S, Takamizawa H, Simizu B
Intervirology. 1986;25(3):151-7. doi: 10.1159/000149669.
Biopsies from human genital lesions in Japan (108 samples), including condyloma acuminata, squamous metaplasia, dysplasia, and cervical cancer, were screened for the presence of human papillomavirus (HPV) 6-, 11-, 16-, and 18-related DNAs by spot hybridization and Southern blot hybridization under stringent conditions. By spot hybridization, HPV 6/11-related DNA was found in 92.9% (13/14) of condyloma acuminata and in 6.7% (1/15) of cervical cancer biopsies. HPV 16/18-related DNA was found in 37.7% (5/14) of cervical cancer biopsies and was exclusively associated with invasive squamous cell cancer, 66.7% (8/12), and with cervical carcinoma in situ, 50% (9/18). Some sample DNAs were further characterized by restriction enzyme digestion followed by Southern blot analysis, and we confirmed the presence of HPV-specific DNA fragments and mixed infection with both HPV 6/11- and HPV 16/18-related HPVs in one lesion.
对来自日本的人体生殖器病变组织活检样本（108份）进行检测，这些样本包括尖锐湿疣、鳞状化生、发育异常和宫颈癌，通过斑点杂交和严格条件下的Southern印迹杂交，筛查人乳头瘤病毒（HPV）6、11、16和18相关DNA的存在情况。通过斑点杂交，在92.9%（13/14）的尖锐湿疣样本和6.7%（1/15）的宫颈癌活检样本中发现了HPV 6/11相关DNA。在37.7%（5/14）的宫颈癌活检样本中发现了HPV 16/18相关DNA，且仅与浸润性鳞状细胞癌（66.7%，8/12）和宫颈原位癌（50%，9/18）相关。一些样本DNA通过限制性内切酶消化后进行Southern印迹分析进一步鉴定，我们证实了HPV特异性DNA片段的存在以及在一个病变中同时感染HPV 6/11和HPV 16/18相关HPV的情况。
