Ohishi H, Shigemoto R, Nakanishi S, Mizuno N
Department of Morphological Brain Science, Faculty of Medicine, Kyoto University, Japan.
J Comp Neurol. 1993 Sep 8;335(2):252-66. doi: 10.1002/cne.903350209.
Distribution of the mRNA for a metabotropic glutamate receptor, mGluR3, which is coupled to the inhibitory cAMP cascade, was examined in the central nervous system of the adult albino rat by in situ hybridization. The hybridization signals of mGluR3 were detected not only on neuronal cells but also on many glial cells throughout the brain and spinal cord. In the neuronal cells, prominent expression of mGluR3 mRNA was seen in the thalamic reticular nucleus. Moderately labeled neurons were seen in the anterior olfactory nucleus, cerebral neo- and mesocortical regions, lateral amygdaloid nucleus, ventral part of the basolateral amygdaloid nucleus, dorsal endopiriform nucleus, supraoptic nucleus, superficial layers of the superior colliculus, inferior colliculus, interpeduncular nucleus, superior olivary nuclei, and Golgi cells in the cerebellar cortex. Weakly labeled neurons were observed in the striatum, nucleus accumbens, ventral pallidum, globus pallidus, entopeduncular nucleus, lateral hypothalamic area, hypothalamic paraventricular nucleus, medial habenular nucleus, anterior pretectal nucleus, Barrington's nucleus, Nucleus O, paragenual nucleus, trigeminal sensory complex, cochlear nuclei, dorsal motor nucleus of the trigeminal nerve, dorsal cap of the inferior olive, spinal dorsal horn, and lamina X of the spinal cord. The stellate cells in the cerebellar cortex, and neurons in the deep cerebellar nuclei were also labeled weakly. The granule cell layer of the dentate gyrus, as a whole, appeared to be labeled intensely, but each of the granule cells was labeled only weakly. No significant labeling was detected in the mitral and tufted cells in the olfactory bulb, hippocampal pyramidal cells, Purkinje and granule cells in the cerebellar cortex, or somatic motoneurons. The distribution of mGluR3 mRNA in particular neurons and glial cells indicates specific roles of mGluR3 in the glutamatergic system of the central nervous system.
通过原位杂交技术，在成年白化大鼠的中枢神经系统中检测了与抑制性环磷酸腺苷（cAMP）级联反应偶联的代谢型谷氨酸受体mGluR3的mRNA分布。mGluR3的杂交信号不仅在神经元细胞上被检测到，而且在整个脑和脊髓的许多胶质细胞上也被检测到。在神经元细胞中，丘脑网状核中可见mGluR3 mRNA的显著表达。在前嗅核、大脑新皮质和中皮质区域、杏仁外侧核、基底外侧杏仁核腹侧部分、背侧梨状内核、视上核、上丘浅层、下丘、脚间核、上橄榄核以及小脑皮质的高尔基细胞中可见中等程度标记的神经元。在纹状体、伏隔核、腹侧苍白球、苍白球、内囊核、下丘脑外侧区、下丘脑室旁核、内侧缰核、前顶盖前核、巴林顿核、O核、旁膝状核、三叉神经感觉复合体、耳蜗核、三叉神经背运动核、下橄榄核背帽、脊髓背角和脊髓X层中观察到弱标记的神经元。小脑皮质的星状细胞和小脑深部核团中的神经元也被弱标记。齿状回的颗粒细胞层总体上似乎被强烈标记，但每个颗粒细胞仅被弱标记。在嗅球的二尖瓣细胞和簇状细胞、海马锥体细胞、小脑皮质的浦肯野细胞和颗粒细胞或躯体运动神经元中未检测到明显的标记。mGluR3 mRNA在特定神经元和胶质细胞中的分布表明mGluR3在中枢神经系统谷氨酸能系统中具有特定作用。
