Kaizu H, Sasaki M, Nakajima H, Suzuki Y
Technical Research Institute, Snow Brand Milk Products Co. Ltd., Kawagoe, Japan.
J Dairy Sci. 1993 Sep;76(9):2493-9. doi: 10.3168/jds.S0022-0302(93)77584-0.
Lactic acid bacteria, including Bifidobacterium, with antioxidative activity were selected by in vitro screening. The effect of the antioxidative activity was investigated by in vivo experiments using rats that were deficient in vitamin E. In the first stage of screening, 570 strains were examined; intracellular cell-free extracts of 19 strains (16 lactobacilli, 2 streptococci, and 1 lactococci) had antioxidative activity as determined by an assay using rat liver microsomes and thiobarbituric acid. In the second stage of screening, 7 strains of lactobacilli showed over 70% inhibition of oxidation activity. The highest activity was obtained by heterofermentative Lactobacillus sp. SBT 2028. The effect of two strains, Lactobacillus sp. SBT 2028 and Lactobacillus casei ssp. rhamnosus SBT 2257, was evaluated for improvement of the condition of rats fed a diet deficient in vitamin E. Intracellular cell-free extracts of those two strains were also used for in vivo experiments. Hemolysis of red blood cells was inhibited in rats that were administered the extract of Lactobacillus sp. SBT 2028, which proved that the extract improved the vitamin E deficiency status. Antioxidative activity of an extract from L. casei ssp. rhamnosus SBT 2257 determined by hemolysis was relatively weak compared with the activity of Lactobacillus sp. SBT 2028 extract.
通过体外筛选，选出了包括双歧杆菌在内具有抗氧化活性的乳酸菌。利用维生素E缺乏的大鼠进行体内实验，研究了其抗氧化活性的效果。在筛选的第一阶段，检测了570株菌株；通过使用大鼠肝微粒体和硫代巴比妥酸的检测方法确定，19株菌株（16株乳酸杆菌、2株链球菌和1株乳酸球菌）的细胞内无细胞提取物具有抗氧化活性。在筛选的第二阶段，7株乳酸杆菌显示出超过70%的氧化活性抑制率。最高活性是由异型发酵乳酸杆菌SBT 2028获得的。评估了两株菌株，即乳酸杆菌SBT 2028和鼠李糖乳杆菌亚种SBT 2257对改善喂食维生素E缺乏饮食的大鼠状况的效果。这两株菌株的细胞内无细胞提取物也用于体内实验。给予乳酸杆菌SBT 2028提取物的大鼠红细胞溶血受到抑制，这证明该提取物改善了维生素E缺乏状况。与乳酸杆菌SBT 2028提取物的活性相比，通过溶血测定的鼠李糖乳杆菌亚种SBT 2257提取物的抗氧化活性相对较弱。
