Arias Padró Maria D, Caboni Emilia, Salazar Morin Karla Azucena, Meraz Mercado Marco Antonio, Olalde-Portugal Víctor
Departamento de Biotecnología y Bioquímica, Centro de Investigación y de Estudios Avanzados del IPN, Irapuato, Guanajuato, Mexico.
Consiglio per la Ricerca in Agricoltura e l 'Analisi dell'Economia Agraria (CREA), Olivicoltura, Frutticoltura e Agrumicoltura (OFA), Rome, Italy.
PeerJ. 2021 Mar 12;9:e10984. doi: 10.7717/peerj.10984. eCollection 2021.
Grafting generally means stress to a plant and this triggers antioxidant defense systems. An imbalance in reactive oxygen species may negatively affect the grafting success. Several research projects have studied the association with plant growth-promoting rhizobacteria (PGPR) and it has been documented that they enhance nutrient acquisition, regulate hormone levels, and influence the antioxidant response in crops. However, little is known about the strategy of inoculating grafted herbaceous plants with PGPR and its effect on the antioxidant response. The effects of inoculating a strain of on the antioxidant metabolism of grafted tomato were evaluated. In this study, two different rootstocks were used for tomato ( L. var. Rio Grande (RG)): [ L. var. cerasiforme (Ch)] and eggplant [( L. (Ber)] to establish a compatible graft (RGCh) and a semicompatible graft (RGBer). Enzyme activities involved in the antioxidant defense system: superoxide dismutase (SOD), catalase (CAT), phenylalanine ammonia lyase (PAL), polyphenol oxidase (PPO), peroxidase (POD), and total phenols were measured during 4 weeks after grafting. The results show that for RGCh, during the first two weeks after grafting, the tendency was a decrease of the enzyme activity for SOD, CAT, PAL when inoculated with ; while in the semicompatible graft RGBer, PPO and PAL decreased their activity after inoculation. For both combinations, the quantity of total phenols varied depending on the day. In both graft combinations, applications of resulted in 86 and 80% callusing compared with the uninoculated control where the percentages were 74 and 70% for RGCh and RGBer, respectively. The highest significant graft success (95%) was recorded 28 days after grafting for inoculated RGBer. These findings imply that induced antioxidant mechanisms in grafted plants and suggest that inoculation with this growth-promoting bacterium can represent a biotechnological approach to improve success in tomato grafting.
嫁接通常意味着对植物造成胁迫，这会触发抗氧化防御系统。活性氧物种的失衡可能会对嫁接成功率产生负面影响。几个研究项目已经研究了与植物促生根际细菌（PGPR）的关联，并且已经证明它们可以增强养分获取、调节激素水平并影响作物的抗氧化反应。然而，关于用PGPR接种嫁接草本植物的策略及其对抗氧化反应的影响知之甚少。评估了接种一种菌株对嫁接番茄抗氧化代谢的影响。在本研究中，两种不同的砧木用于番茄（L. var. Rio Grande（RG））：[L. var. cerasiforme（Ch）]和茄子[(L.（Ber）)]，以建立一个兼容嫁接（RGCh）和一个半兼容嫁接（RGBer）。在嫁接后的4周内测量了抗氧化防御系统中涉及的酶活性：超氧化物歧化酶（SOD）、过氧化氢酶（CAT）、苯丙氨酸解氨酶（PAL）、多酚氧化酶（PPO）、过氧化物酶（POD）和总酚。结果表明，对于RGCh，在嫁接后的前两周，接种时SOD、CAT、PAL的酶活性呈下降趋势；而在半兼容嫁接RGBer中，接种后PPO和PAL的活性下降。对于这两种组合，总酚的量因天数而异。在两种嫁接组合中，与未接种对照相比，接种的愈伤组织形成率分别为86%和80%，RGCh和RGBer未接种对照的愈伤组织形成率分别为74%和70%。接种的RGBer在嫁接后28天记录到最高的显著嫁接成功率（95%）。这些发现意味着诱导了嫁接植物中的抗氧化机制，并表明接种这种促生长细菌可以代表一种生物技术方法来提高番茄嫁接的成功率。
