Dogra R K, Khanna S, Srivastava S N, Shukla L J, Chandra K, Saxena G, Shanker R
Immunotoxicology Laboratory, Industrial Toxicology Research Centre, Lucknow, India.
Immunopharmacol Immunotoxicol. 1993 Aug;15(4):491-514. doi: 10.3109/08923979309035242.
Pathomorphological and immunological alterations caused by a mixture of styrene and dioctyl phthalate were studied in albino mice following oral administration of 0.02, 0.03, 0.05 x LD50 of the mixture. The chemicals were mixed together proportionate to their respective LD50 values and fed in ground nut oil, 5 d/wk for 4 weeks. Histological examination of spleen revealed considerable depletion of cellular population of lymphoid follicles which corresponded to the dose dependent decrease in splenic mononuclear cell population count. The thymic lobules revealed slight atrophy but accompanied by a significant increase in thymocyte population. Correspondingly few significant histological changes were observed in mesenteric and peripheral lymph nodes. The treatment caused impairment of primary humoral immune response to SRBC (IgM) but there was a significant increase in response of splenocytes to B-cell mitogen LPS. There was a suppression of cutaneous delayed type hypersensitivity and increase in splenic lymphocyte response to T-cell mitogen PHA. Simultaneously, indirect immunity represented by decreased phagocytosis and enhanced metabolic function of reducing NBT by peritoneal exudate cells was observed. The in vitro exposure of vero cells to the mixture caused dose dependent protective effect. The results of present study indicate that subchronic exposure to low doses of mixture of styrene and dioctyl phthalate under certain conditions may modulate some of the immune functions as compared to exposure to either chemicals alone.
在白化小鼠口服0.02、0.03、0.05倍半数致死剂量（LD50）的苯乙烯和邻苯二甲酸二辛酯混合物后，研究了该混合物引起的病理形态学和免疫学改变。将这些化学物质按各自LD50值的比例混合，溶于花生油中，每周5天，连续喂饲4周。脾脏组织学检查显示，淋巴滤泡的细胞数量显著减少，这与脾单核细胞数量的剂量依赖性减少相对应。胸腺小叶显示轻度萎缩，但胸腺细胞数量显著增加。相应地，在肠系膜淋巴结和外周淋巴结中观察到的显著组织学变化较少。该处理导致对绵羊红细胞（SRBC）的初次体液免疫反应（IgM）受损，但脾细胞对B细胞有丝分裂原脂多糖（LPS）的反应显著增加。皮肤迟发型超敏反应受到抑制，脾淋巴细胞对T细胞有丝分裂原植物血凝素（PHA）的反应增强。同时，观察到以吞噬作用降低和腹膜渗出细胞还原硝基蓝四唑（NBT）的代谢功能增强为代表的间接免疫。体外将vero细胞暴露于该混合物会产生剂量依赖性保护作用。本研究结果表明，与单独暴露于任何一种化学物质相比，在某些条件下亚慢性暴露于低剂量的苯乙烯和邻苯二甲酸二辛酯混合物可能会调节某些免疫功能。
