Ovcinnikov N M, Delektorskij V V, Dmitriev G A
Br J Vener Dis. 1976 Aug;52(4):230-45. doi: 10.1136/sti.52.4.230.
The authors have made an electron microscope study of material from patients with acute, chronic, and asymptomatic gonorrhoea both before and during treatment with penicillin and kanamycin. Some features of the structure of the gonococcus, e.g. the six-layered cell wall, and of the reactions of the organism in different forms of gonorrhoeae before and during treatment with antibiotics were demonstrated using Ruthenium red staining. Thus, in chronic gonorrhoea, the presence of structures similar to gonococcal L-forms was found to be characteristic, while in asymptomatic gonorrhoea the formation was observed of multimembrane phagosomes hampering the penetration of drugs and antibodies. The positioning of gonococci round epithelial cells was noted (cellular energy). The possibility of changing treatment procedures is examined in the light of these observations.
作者对急性、慢性和无症状淋病患者在青霉素和卡那霉素治疗前及治疗期间的材料进行了电子显微镜研究。使用钌红染色展示了淋球菌的一些结构特征，如六层细胞壁，以及该生物体在不同形式淋病中治疗前和治疗期间对抗生素的反应。因此，发现在慢性淋病中，存在类似于淋球菌L型的结构是其特征，而在无症状淋病中，观察到形成了多膜吞噬体，阻碍了药物和抗体的渗透。注意到淋球菌围绕上皮细胞的定位（细胞活力）。根据这些观察结果探讨了改变治疗程序的可能性。
