Myrvik A L, Whitaker J M, Cannon R E
Can J Microbiol. 1976 Jul;22(7):1002-6. doi: 10.1139/m76-146.
The use of agar in media for culturing microorganisms is fundamental to microbiological investigations. Shortages of agar have caused increased costs and difficulty in obtaining media. Evidence is presented for the use of carboxymethylcellulose (CMC), an inert compound, in conjunction with agar to reduce the concentration of agar necessary to achieve a solid plating surface. A variety of bacteria, blue-green bacteria, fungi, and a yeast were tested for growth on CMC agar media. T-2 bacteriophage and three cyanophages were tested for plaque-forming efficiency on CMC agar plates. Selective and differential media were also formulated with a CMC agar supplement. Growth of all microorganisms was comparable on CMC and agar control. Use of cellulose products provides a means of decreasing agar consumption without affecting successful cultivation of microorganisms.
在用于培养微生物的培养基中使用琼脂是微生物学研究的基础。琼脂短缺导致培养基成本增加且获取困难。本文提供了证据，表明使用惰性化合物羧甲基纤维素（CMC）与琼脂结合，可降低获得固体平板表面所需的琼脂浓度。测试了多种细菌、蓝细菌、真菌和一种酵母在CMC琼脂培养基上的生长情况。测试了T-2噬菌体和三种蓝藻噬菌体在CMC琼脂平板上的噬菌斑形成效率。还配制了添加CMC琼脂的选择性和鉴别培养基。所有微生物在CMC和琼脂对照上的生长情况相当。使用纤维素产品提供了一种减少琼脂消耗而又不影响微生物成功培养的方法。
