Jakucs E, Várallyay E
Department of Plant Anatomy, Loránd Eötvös University Budapest, Hungary.
Acta Microbiol Immunol Hung. 1995;42(1):77-80.
A simple and rapid semiquantitative technique for the determination of fungal cellulytic activities in solid (agar slant) media has been developed. This method is a combination of Congo-red staining widely used for qualitative cellulase detection and common cellulase activity tests. Previous investigation on the adsorptive effect of cellulose content of media showed that the real enzyme activity values can be measured with minimum loss by means of agar discs cut from the most active zones of slants visualized by Congo-red staining. Different cellulase activity tests (FPase, CMCase and beta-glucosidase by PNPG-method) of seven cellulolytic fungal strains were investigated by this technique. Data give information on the different enzyme profiles of the species. The method can be regarded as very simple and suitable for simultaneous rapid comparison of cellulase components of greater series of fungal strains from agar slant cultures. It can also be used in the case of fungi unable to grow in liquid cultures.
已开发出一种用于测定固体（琼脂斜面）培养基中真菌纤维素分解活性的简单快速半定量技术。该方法是广泛用于定性纤维素酶检测的刚果红染色法与常见纤维素酶活性测试的结合。先前关于培养基纤维素含量吸附作用的研究表明，通过从刚果红染色显示的斜面最活跃区域切下的琼脂圆盘，可以以最小的损失测量实际酶活性值。用该技术研究了七种纤维素分解真菌菌株的不同纤维素酶活性测试（滤纸酶、羧甲基纤维素酶和通过对硝基苯-β-D-葡萄糖苷法测定的β-葡萄糖苷酶）。数据给出了这些物种不同酶谱的信息。该方法可被视为非常简单，适用于同时快速比较来自琼脂斜面培养物的大量真菌菌株的纤维素酶成分。它也可用于无法在液体培养物中生长的真菌。
