Irrgang S, Baumgärtl H, Schlosser D, Zimelka W, Schmauder H P
Institut für Mikrobiologie, Universität Jena, Dortmund, Germany.
J Basic Microbiol. 1993;33(5):311-21. doi: 10.1002/jobm.3620330506.
The oxygen supply of free, Ca-alginate entrapped and microencapsulated mycelia of Penicillium raistrickii i 477 capable of 15 alpha-hydroxylation of 13-ethyl-gon-4-en-3,17-dione was investigated. Using an oxygen microelectrode distinct gradients of oxygen within the Ca-alginate beads as well as the microcapsules were detected. Slope and width of the gradients were investigated in dependence on the kind of immobilization, the culture age and the cell density on or in the carrier as well as the different forms of the oxygen supply in the medium. So it could be shown that large parts of immobilizates, approximately 96% of the diameter of both types, were oxygen-free. In comparison with free mycelia, the lower oxygen supply of the immobilized mycelia led to a metabolic shift to fermentative catabolism.
对能够对13-乙基-4-烯-3,17-二酮进行15α-羟基化的雷斯垂克青霉i 477的游离、海藻酸钙包埋和微囊化菌丝体的氧气供应进行了研究。使用氧微电极检测到海藻酸钙珠以及微囊内存在明显的氧梯度。根据固定化类型、培养年龄、载体上或载体中的细胞密度以及培养基中不同的氧气供应形式，对梯度的斜率和宽度进行了研究。结果表明，大部分固定化产物，即两种类型直径的约96%，是无氧的。与游离菌丝体相比，固定化菌丝体较低的氧气供应导致代谢转变为发酵分解代谢。
