McClelland D B, Van Furth R
Clin Exp Immunol. 1976 Sep;25(3):442-8.
The "skin window" technique in which the horny layer of the skin is abraded with a high speed grinder has been used to study the appearance of proteins with antimicrobial activity in the fluid accumulating in damaged human skin. The fluid was absorbed into paper discs and protein levels measured by radial diffusion. The skin exudates contained about 45% as much IgG and IgM as the subjects' serum, but the amount of IgA (68% of the serum level) in the exudate was significantly greater, suggesting selective transport into the lesion. The fluid also contains complement proteins, lysozyme and lactoferrin. The methods used in this study may provide useful information about clinical situations in which susceptibility to cutaneous infection is increased.
“皮肤窗口”技术是用高速研磨机磨损皮肤角质层，已被用于研究在受损人类皮肤中积聚的液体里具有抗菌活性的蛋白质的出现情况。将该液体吸收到纸碟中，通过径向扩散测量蛋白质水平。皮肤渗出液中所含的IgG和IgM约为受试者血清的45%，但渗出液中IgA的量（血清水平的68%）显著更高，表明有选择性地转运至损伤部位。该液体还含有补体蛋白、溶菌酶和乳铁蛋白。本研究中使用的方法可能会为皮肤感染易感性增加的临床情况提供有用信息。
