Badinand F, Fontbonne A, Maurel M C, Siliart B
Ecole Nationale Vétérinaire, Maisons-Alfort, France.
J Reprod Fertil Suppl. 1993;47:63-7.
Seven bitches of several breeds were monitored during oestrus by vaginal smears and luteinizing hormone (LH), oestradiol and progesterone plasma assays. Each bitch was inseminated into the uterus with frozen spermatozoa from seven dogs of different breeds. Each female was inseminated daily with about 200 x 10(6) motile spermatozoa. Six bitches delivered. The paternity of puppies (which indicated the fertilization time) was determined by morphological analysis. Two bitches were fertilized with one artificial insemination (AI) only and four bitches with two successive AIs. Fertilization occurred 84-180 h after the LH peak and 1-3 days before the first day of metoestrus. The gestation length, from fertilization to birth, was 58-63 days or 56-60 days after the first day of metoestrus.
通过阴道涂片以及黄体生成素（LH）、雌二醇和孕酮血浆检测，对几只不同品种的母犬在发情期进行了监测。每只母犬都用来自七只不同品种公犬的冷冻精子进行子宫内授精。每天给每只母犬输入约2亿活动精子。六只母犬分娩。通过形态学分析确定幼犬的父系（这表明了受精时间）。两只母犬仅接受一次人工授精（AI），四只母犬接受连续两次人工授精。受精发生在LH峰值后84 - 180小时以及发情后期第一天前1 - 3天。从受精到分娩的妊娠期为58 - 63天，或发情后期第一天后56 - 60天。
