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白细胞介素-2通过转录后机制下调转基因小鼠中的乙型肝炎病毒基因表达。

Interleukin-2 downregulates hepatitis B virus gene expression in transgenic mice by a posttranscriptional mechanism.

作者信息

Guilhot S, Guidotti L G, Chisari F V

机构信息

Department of Molecular and Experimental Medicine, Scripps Research Institute, La Jolla, California 92037.

出版信息

J Virol. 1993 Dec;67(12):7444-9. doi: 10.1128/JVI.67.12.7444-7449.1993.

DOI:10.1128/JVI.67.12.7444-7449.1993
PMID:8230465
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC238210/
Abstract

We have recently demonstrated that tumor necrosis factor alpha (TNF-alpha) and interleukin-2 (IL-2) downregulate the hepatic steady-state content of hepatitis B virus (HBV) mRNA in vivo in HBV-transgenic mice and that the IL-2 effect is mediated by TNF-alpha. In the current study, we demonstrate that IL-2-induced downregulation of hepatic HBV 2.1-kb mRNA is not due to changes in the transcription rate or the intranuclear maturation or export of this transcript but that it is selectively and profoundly depleted from the cytoplasm of the liver cells in vivo following IL-2 administration. Collectively, these results suggest that IL-2 alters the steady-state content of hepatic HBV mRNA by a posttranscriptional mechanism in vivo, that this effect is mediated by TNF-alpha, and that it probably reflects increased cytoplasmic degradation of the viral transcript.

摘要

我们最近证明，肿瘤坏死因子α（TNF-α）和白细胞介素-2（IL-2）在体内可下调乙型肝炎病毒（HBV）转基因小鼠肝脏中HBV mRNA的稳态含量，且IL-2的作用是由TNF-α介导的。在本研究中，我们证明IL-2诱导的肝脏HBV 2.1-kb mRNA下调并非由于该转录本的转录速率、核内成熟或输出发生改变，而是在体内给予IL-2后，它从肝细胞的细胞质中被选择性地大量清除。总体而言，这些结果表明，IL-2在体内通过转录后机制改变肝脏HBV mRNA的稳态含量，该作用由TNF-α介导，并且这可能反映了病毒转录本在细胞质中的降解增加。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7d4/238210/b5ff463d3383/jvirol00033-0546-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7d4/238210/267e6fc5bc8a/jvirol00033-0544-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7d4/238210/434596cdc9fa/jvirol00033-0545-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7d4/238210/b5ff463d3383/jvirol00033-0546-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7d4/238210/267e6fc5bc8a/jvirol00033-0544-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7d4/238210/434596cdc9fa/jvirol00033-0545-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/d7d4/238210/b5ff463d3383/jvirol00033-0546-a.jpg

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