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核因子-κB p100是在转化的鸡脾细胞中与v-Rel癌蛋白复合的高分子量蛋白质之一。

NF-kappa B p100 is one of the high-molecular-weight proteins complexed with the v-Rel oncoprotein in transformed chicken spleen cells.

作者信息

Sif S, Gilmore T D

机构信息

Department of Biology, Boston University, Massachusetts 02215.

出版信息

J Virol. 1993 Dec;67(12):7612-7. doi: 10.1128/JVI.67.12.7612-7617.1993.

DOI:10.1128/JVI.67.12.7612-7617.1993
PMID:8230480
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC238228/
Abstract

The Rel/NF-kappa B family of proteins includes several interacting cellular transcription factors and the v-Rel oncoprotein of the avian Rev-T retrovirus. We report the isolation of a chicken cDNA for the NF-kappa B p52 precursor protein p100. Full-length p100 only weakly binds DNA in vitro; removal of the ankyrin-like repeats generates C-terminally truncated p100 proteins (like p52) that have an increased ability to bind an oligonucleotide containing a kappa B site. In addition, we show that chicken p100 is identical to a protein previously designated p115, which is found in a complex with v-Rel in transformed chicken spleen cells. Furthermore, p100 and v-Rel can form a complex when synthesized in vitro. Using cDNAs for chicken NF-kappa B p105, NF-kappa B p100, c-Rel, and v-Rel, we show that one of the complexes in v-Rel-transformed spleen cells can be reconstituted in vitro.

摘要

Rel/NF-κB 蛋白家族包括几种相互作用的细胞转录因子以及禽 Rev-T 逆转录病毒的 v-Rel 癌蛋白。我们报告了鸡 NF-κB p52 前体蛋白 p100 的 cDNA 的分离。全长 p100 在体外仅微弱结合 DNA；去除锚蛋白样重复序列会产生 C 末端截短的 p100 蛋白（如 p52），其结合含有 κB 位点的寡核苷酸的能力增强。此外，我们表明鸡 p100 与先前命名为 p115 的蛋白相同，该蛋白在转化的鸡脾细胞中与 v-Rel 形成复合物。此外，p100 和 v-Rel 在体外合成时可形成复合物。利用鸡 NF-κB p105、NF-κB p100、c-Rel 和 v-Rel 的 cDNA，我们表明 v-Rel 转化的脾细胞中的一种复合物可以在体外重建。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d58/238228/e898bbab700d/jvirol00033-0714-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d58/238228/4a99c11ebacd/jvirol00033-0713-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d58/238228/ea8672baeed3/jvirol00033-0713-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d58/238228/e898bbab700d/jvirol00033-0714-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d58/238228/4a99c11ebacd/jvirol00033-0713-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d58/238228/ea8672baeed3/jvirol00033-0713-b.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/2d58/238228/e898bbab700d/jvirol00033-0714-a.jpg

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本文引用的文献

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Genes Dev. 1993 Apr;7(4):705-18. doi: 10.1101/gad.7.4.705.
2
A conditional mutant of vRel containing sequences from the human estrogen receptor.一种含有来自人雌激素受体序列的vRel条件突变体。
Virology. 1993 Mar;193(1):160-70. doi: 10.1006/viro.1993.1112.
3
v-Rel and c-Rel are differentially affected by mutations at a consensus protein kinase recognition sequence.v-Rel和c-Rel受共有蛋白激酶识别序列处突变的影响不同。
J Cardiovasc Transl Res. 2010 Aug;3(4):344-54. doi: 10.1007/s12265-010-9195-5. Epub 2010 May 25.
4
Mutational analysis of the v-Rel dimerization interface reveals a critical role for v-Rel homodimers in transformation.对v-Rel二聚化界面的突变分析揭示了v-Rel同型二聚体在转化过程中的关键作用。
J Virol. 2002 May;76(10):4928-39. doi: 10.1128/jvi.76.10.4928-4939.2002.
5
Mapping of a serine-rich domain essential for the transcriptional, antiapoptotic, and transforming activities of the v-Rel oncoprotein.对v-Rel癌蛋白转录、抗凋亡和转化活性至关重要的富含丝氨酸结构域的定位
Mol Cell Biol. 1999 Jan;19(1):307-16. doi: 10.1128/MCB.19.1.307.
6
I kappa B epsilon, a novel member of the I kappa B family, controls RelA and cRel NF-kappa B activity.IκBε，IκB家族的一个新成员，控制RelA和cRel核因子κB的活性。
EMBO J. 1997 Mar 17;16(6):1413-26. doi: 10.1093/emboj/16.6.1413.
7
Interaction of the v-Rel oncoprotein with NF-kappaB and IkappaB proteins: heterodimers of a transformation-defective v-Rel mutant and NF-2 are functional in vitro and in vivo.v-Rel癌蛋白与NF-κB和IκB蛋白的相互作用：转化缺陷型v-Rel突变体与NF-2的异二聚体在体外和体内均具有功能。
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8
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J Virol. 1994 Jan;68(1):308-19. doi: 10.1128/JVI.68.1.308-319.1994.
9
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5
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6
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Mol Cell Biol. 1993 Jul;13(7):3964-74. doi: 10.1128/mcb.13.7.3964-3974.1993.
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