Mizusawa T
Nihon Naibunpi Gakkai Zasshi. 1975 Dec 20;51(12):997-1014. doi: 10.1507/endocrine1927.51.12_997.
Crude gonadotropins extracted from the urine of patients with chorioepithelioma (choriocarcinoma) by Bradbury method was purified by a combination of Sephadex gel filtration, CM-C and DEAE-C chromatography. Two biologically active fractions (fraction t-hCG-A and fraction t-hCG-C) were obtained. The former cross-reacted with anti-hCG sera and the latter cross-reacted with anti-hCFSH sera in experiments with Ouchterlony immunodiffusion and immunoelectrophoresis. But the hCG or hCFSH from normal pregnancy and t-hCG-C from choriocarcinoma were different in their potency of being adsorbed on ion-exchange cellulose respectively. T-hCG-C was poorer in aspartic acid and glycine, and richer in serine, threonine and tyrosin to which carbohydrates bind than hCFSH from normal pregnancy. These two fractions contained high concentrations of carbohydrates, especially hexose and sialic acid, less concentration of hexosamine, compared to those of hCG and hCFSH obtained from urine of normal pregnant women. Sera of normal pregnant women in the first and third trimester and those of patients with chorionic neoplasias were gel filtrated on a Sephadex G-100 upward flow column in the same conditions. Biological activity of hCG in sera of normal pregnant women was recognized in 2-3 peaks on the gel filtration, and the molecular weights of those were considered to be about 25,000-40,000. In case of sera of chorionic neoplasias, however, it was admitted as multi-peaks (the molecular weights: about 10,000-70,000). It might be one of the features of chorionic neoplasias that the biological activity was found even in fractions of molecular weight 10,000 on the gel filtration, and to pay attention to this phenomenon might be a useful sign for the diagnosis or the management of patients with chorionic neoplasias. As a conclusion, all the above findings suggest that the molecular structure of t-hCG-A and t-hCG-C from choriocarcinoma differes from that of hCG and hCFSH from normal pregnancy respectively.
采用布拉德伯里方法从绒毛膜上皮癌（绒毛膜癌）患者尿液中提取的粗制促性腺激素，通过葡聚糖凝胶过滤、CM - C和DEAE - C色谱法相结合的方式进行纯化。获得了两个具有生物活性的组分（组分t - hCG - A和组分t - hCG - C）。在双向免疫扩散和免疫电泳实验中，前者与抗hCG血清发生交叉反应，后者与抗hCFSH血清发生交叉反应。但是，正常妊娠的hCG或hCFSH以及绒毛膜癌的t - hCG - C在离子交换纤维素上的吸附能力有所不同。与正常妊娠的hCFSH相比，t - hCG - C的天冬氨酸和甘氨酸含量较低，而丝氨酸、苏氨酸和酪氨酸含量较高，碳水化合物与之结合。这两个组分含有高浓度的碳水化合物，尤其是己糖和唾液酸，与正常孕妇尿液中获得的hCG和hCFSH相比，氨基己糖浓度较低。在相同条件下，对妊娠早期和晚期正常孕妇以及绒毛膜肿瘤患者的血清在葡聚糖G - 100向上流动柱上进行凝胶过滤。正常孕妇血清中hCG的生物活性在凝胶过滤时有2 - 3个峰被识别，其分子量约为25,000 - 40,000。然而，对于绒毛膜肿瘤患者的血清，其表现为多个峰（分子量约为10,000 - 70,000）。凝胶过滤时在分子量10,000的组分中仍发现生物活性可能是绒毛膜肿瘤的特征之一，关注这一现象可能对绒毛膜肿瘤患者的诊断或治疗具有重要意义。总之，上述所有发现表明，绒毛膜癌的t - hCG - A和t - hCG - C的分子结构分别与正常妊娠的hCG和hCFSH不同。
