Ohta H, Matsumoto K, Watanabe H, Shimizu M
Section of Pharmacology, Research Institute for Wakan-Yaku, Toyama, Japan.
Jpn J Pharmacol. 1993 Aug;62(4):345-9. doi: 10.1254/jjp.62.345.
Paeoniflorin, a major constituent of peony root, has been demonstrated to attenuate the radial maze performance deficit produced by scopolamine. In the present study, to investigate the possible involvement of beta-adrenergic systems in the paeoniflorin antagonism of the scopolamine deficit, the effects of two beta-adrenoceptor antagonists, propranolol and atenolol, on the paeoniflorin effect were examined in male Wistar rats. Paeoniflorin (1 mg/kg, p.o.) significantly attenuated the scopolamine HBr (0.3 mg/kg, i.p.)-induced deficit in the choice accuracy in radial maze performance without changing the running time prolonged by scopolamine. Neither D,L-propranolol HCl, a lipophilic beta-antagonist, at 3 mg/kg, i.p. nor atenolol, a hydrophilic beta 1-antagonist that is known to hardly ever cross the blood-brain barrier, at 1 mg/kg, i.p. impaired maze performance by itself or aggravated the scopolamine-induced deficit in radial maze performance. Both antagonists, however, completely blocked the antagonizing effect of paeoniflorin on the scopolamine deficit. These data suggest that the beta-adrenergic systems, especially peripheral beta 1-adrenergic systems, are involved in the antagonizing effect of paeoniflorin on the scopolamine deficit in radial maze performance in rats.
芍药苷是芍药根的主要成分，已被证明可减轻东莨菪碱引起的放射状迷宫行为缺陷。在本研究中，为了探究β-肾上腺素能系统是否参与芍药苷对东莨菪碱缺陷的拮抗作用，在雄性Wistar大鼠中检测了两种β-肾上腺素能受体拮抗剂普萘洛尔和阿替洛尔对芍药苷作用的影响。芍药苷（1毫克/千克，口服）显著减轻了氢溴酸东莨菪碱（0.3毫克/千克，腹腔注射）诱导的放射状迷宫选择准确性缺陷，且未改变东莨菪碱延长的奔跑时间。脂溶性β-拮抗剂盐酸普萘洛尔（3毫克/千克，腹腔注射）和已知几乎不能穿过血脑屏障的亲水性β1-拮抗剂阿替洛尔（1毫克/千克，腹腔注射）单独使用时均未损害迷宫行为，也未加重东莨菪碱诱导的放射状迷宫行为缺陷。然而，这两种拮抗剂均完全阻断了芍药苷对东莨菪碱缺陷的拮抗作用。这些数据表明，β-肾上腺素能系统，尤其是外周β1-肾上腺素能系统，参与了芍药苷对大鼠放射状迷宫中东莨菪碱缺陷的拮抗作用。
