Isenberg H D, Wiener S L, Isenberg G A, Sampson-Scherer J, Urivetzky M, Berkman J I
Infect Immun. 1976 Aug;14(2):483-9. doi: 10.1128/iai.14.2.483-489.1976.
Polyvinyl sponges were implanted subcutaneously on both sides of young female rats. One sponge was infected with 10(8) of either Escherichia coli K-12 F-, Staphylococcus aureus ATCC 25923, or Pseudomonas aeruginosa CDC 7725. P. aeruginosa remained at the inoculum level and S. aureus declined by 1 log, whereas E. coli was reduced 1,000-fold. Only P. aeruginosa was recovered from the blood in 36% of the animals in 24 h and in 20% of the rats in 48 h. The nutrient potential of rat inflammatory fluid was compared to nutrient broth by growth of each bacterium in untreated and heat-inactivated sponge fluids and Trypticase soy broth.
将聚乙烯海绵皮下植入年轻雌性大鼠的两侧。其中一块海绵接种10⁸的大肠杆菌K - 12 F⁻、金黄色葡萄球菌ATCC 25923或铜绿假单胞菌CDC 7725。铜绿假单胞菌数量维持在接种水平，金黄色葡萄球菌数量下降1个对数级，而大肠杆菌数量减少1000倍。仅在24小时内，36%的动物血液中可检测到铜绿假单胞菌，48小时内，20%的大鼠血液中可检测到该菌。通过观察每种细菌在未处理和热灭活的海绵液以及胰蛋白酶大豆肉汤中的生长情况，比较大鼠炎性液与营养肉汤的营养潜力。
