Lewis M A, Frye L D, Gibbs C J, Chou S M, Cutchins E C, Gajdusek D C, Ward G
Infect Immun. 1976 Sep;14(3):759-66. doi: 10.1128/iai.14.3.759-766.1976.
Two herpes-like viruses were isolated from capuchin monkey (Cebus apella) brain and (Cebus albifrons) spleen cell cultures, respectively. Both isolates induced similar cytopathic effects consisting of rounded and ballooned cells in the original monkey cell cultures and in a wide range of permissive cell types. Neutralizing antibody to each virus was present in serum from the capuchin monkey from which it was isolated, but the two viruses did not cross-react by neutralization. Fluorescein isothiocyanate conjugates of hyperimmune rabbit serum to one of the isolates showed an antigenic cross relationship between the two isolates. By electron microscopy, herpes-like virus particles were observed in the nucleus and cytoplasm of infected human diploid fibroblast cell cultures. Virus-infected cell cultures stained with acridine orange revealed small deoxyribonucleic acid-containing intranuclear inclusion bodies. Both viruses were inhibited by 5-fluorodeoxyuridine and inactivated by chloroform or exposure to 56 degrees C for 30 min. Antisera prepared against 16 prototype herpesviruses and cytomegaloviruses did not neutralize approximately 100 50% tissue culture infective doses of either capuchin isolate. Neutralizing antibody to the capuchin isolates was detected in sera from 8 of 17 capuchin monkeys but not in sera from 16 humans, 15 chimpanzees, and 10 spider, 6 rhesus, and 5 squirrel monkeys.
分别从卷尾猴（僧帽猴）脑和白额卷尾猴脾细胞培养物中分离出两种疱疹样病毒。两种分离株在原代猴细胞培养物以及多种允许性细胞类型中均诱导出相似的细胞病变效应，包括细胞变圆和气球样变。分离出病毒的卷尾猴血清中存在针对每种病毒的中和抗体，但两种病毒通过中和试验不发生交叉反应。针对其中一种分离株的超免疫兔血清的异硫氰酸荧光素结合物显示，两种分离株之间存在抗原交叉关系。通过电子显微镜观察，在感染的人二倍体成纤维细胞培养物的细胞核和细胞质中发现了疱疹样病毒颗粒。用吖啶橙染色的病毒感染细胞培养物显示出含脱氧核糖核酸的小核内包涵体。两种病毒均被5-氟脱氧尿苷抑制，并被氯仿灭活或在56℃下暴露30分钟后失活。针对16种疱疹病毒原型和巨细胞病毒制备的抗血清不能中和约100个50%组织培养感染剂量的任一卷尾猴分离株。在17只卷尾猴中的8只血清中检测到针对卷尾猴分离株的中和抗体，但在16名人类、15只黑猩猩以及10只蜘蛛猴、6只恒河猴和5只松鼠猴的血清中未检测到。
