Rossmanith W, Karwan R
Institut für Tumorbiologie-Krebsforschung der Universität Wien, Austria.
Mol Biol Rep. 1993 Jun;18(1):29-35. doi: 10.1007/BF01006892.
We show that the Th/To ribonucleoprotein is defined by (i) the co-immunoprecipitation of two RNAs, (ii) the co-immunoprecipitation of four major polypeptides and (iii) the quantitative immune recognition of both RNase P and RNase MRP. No serum was found that recognizes either one of these two enzymes exclusively. The specific co-immunoprecipitation of RNase MRP and RNase P by all Th/To ribonucleoprotein autoantibodies indicates that the anti-Th/To autoimmune response is directed against both enzymes in a quantitatively indistinguishable manner. Thus the Th/To ribonucleoprotein is defined by RNase P and RNase MRP.
我们发现，Th/To核糖核蛋白由以下特征定义：（i）两种RNA的共免疫沉淀；（ii）四种主要多肽的共免疫沉淀；以及（iii）对核糖核酸酶P和核糖核酸酶MRP的定量免疫识别。未发现仅识别这两种酶之一的血清。所有Th/To核糖核蛋白自身抗体对核糖核酸酶MRP和核糖核酸酶P的特异性共免疫沉淀表明，抗Th/To自身免疫反应以定量上无法区分的方式针对这两种酶。因此，Th/To核糖核蛋白由核糖核酸酶P和核糖核酸酶MRP定义。
