使用抗人μ抗体-聚丙烯酰胺珠免疫吸附剂从正常血清中分离大鼠和兔IgM
Isolation of rat and rabbit IgM from normal serum using anti-human mu antibody-polyacrylamide beads immunosorbents.
作者信息
Sapin C, Druet P
出版信息
J Immunol Methods. 1976;12(3-4):355-63. doi: 10.1016/0022-1759(76)90057-0.
PMID:823261
Abstract
The antigenic relationship between human IgM and IgM from other species has been utilized in isolating rat and rabbit IgM from normal sera by means of an anti-human mu-polyacrylamide beads immunoadsorbent. The isolated IgM was found to be pure by immunoelectrophoretic analysis and Ouchterlony technique. The antisera obtained were monospecific for mu-chains after absorption with IgG. Lastly, the IgM isolated always retained its antibody activity.
摘要
人类IgM与其他物种的IgM之间的抗原关系已被用于通过抗人μ-聚丙烯酰胺珠免疫吸附剂从正常血清中分离大鼠和兔的IgM。通过免疫电泳分析和欧氏免疫扩散技术发现，分离出的IgM是纯的。用IgG吸收后获得的抗血清对μ链具有单特异性。最后，分离出的IgM始终保留其抗体活性。
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