Derr R, Zieve L
J Nutr. 1976 Oct;106(10):1398-403. doi: 10.1093/jn/106.10.1398.
Underfed rats infused intravenously with a glucose-amino acid solution at the rate of 390 kcal/kg/day developed a syndrome of muscular weakness, neuropathy, lethargy and precoma or coma associated with severe hypophosphatemia. The movement of phosphate into the cells was studied to determine where it went and into which organic compounds it was incorporated. All but 8% of the labeled phosphate was found in liver, muscle, bone, and carcass residue. Liver cells took up as much phosphate as bone and twice as much as muscle, on weight basis. About 90% of the labeled phosphate entering liver was found in the acid-soluble fraction. The specific activity of liver phosphate increased in the infused underfed rats compared to uninfused underfed rats. Infusion of the underfed rat until signs of the syndrome appeared was associated with a 2.7- to 5-fold increase over the correspondingly infused normal rat in the labeling of glucose-6-phosphate, glucose-1-phosphate, and 6-phosphogluconate. No increase over the infused normal rat was observed in most of the other sugar phosphate compounds nor in the non-sugar phosphate compounds such as phospholipids, nucleic acids or proteins. he changes in sugar phosphates observed in the underfed rats probably reflect the enzymatic atrophy associated with underfeeding and the consequent inability to respond to the huge glucose load.
以390千卡/千克/天的速率静脉输注葡萄糖 - 氨基酸溶液的营养不良大鼠出现了肌肉无力、神经病变、嗜睡和与严重低磷血症相关的前驱昏迷或昏迷综合征。研究了磷酸盐进入细胞的过程，以确定其去向以及它被整合到哪些有机化合物中。除8%外，所有标记的磷酸盐都存在于肝脏、肌肉、骨骼和尸体残渣中。以重量计，肝细胞摄取的磷酸盐与骨骼一样多，是肌肉的两倍。进入肝脏的标记磷酸盐中约90%存在于酸溶性部分。与未输注的营养不良大鼠相比，输注的营养不良大鼠肝脏磷酸盐的比活性增加。将营养不良大鼠输注至出现综合征迹象时，其葡萄糖 - 6 - 磷酸、葡萄糖 - 1 - 磷酸和6 - 磷酸葡萄糖酸的标记量比相应输注正常大鼠增加了2.7至5倍。在大多数其他糖磷酸盐化合物以及非糖磷酸盐化合物（如磷脂、核酸或蛋白质）中，未观察到比输注正常大鼠增加的情况。在营养不良大鼠中观察到的糖磷酸盐变化可能反映了与营养不良相关的酶萎缩以及随后对大量葡萄糖负荷无反应的能力。
