Shimazaki J, Tsubota K, Hayashi K, Kenyon K R, Laing R A
Department of Ophthalmology, Boston University School of Medicine, Mass.
Ophthalmic Res. 1993;25(4):220-5. doi: 10.1159/000267317.
Autofluorescence from reduced pyridine nucleotides (PN) and oxidized flavoproteins (Fp) was measured in order to detect the difference in redox states in rabbit corneal epithelium. The enucleated rabbit eye was mounted in an eye bank eye container with McCarey-Kaufman medium, and the autofluorescence was measured using ocular redox fluorometry as a function of depth. The PN signal distributed evenly whereas the Fp signal was greater in the posterior epithelial region than in the anterior region (p < 0.05). The PN/Fp ratio, a sensitive indicator of tissue redox state, was less in the posterior region. After the application of 1 mM of potassium cyanide in the medium, the ratio increased significantly in each layer (p < 0.001), and the difference between anterior and posterior region diminished. These results indicate that ocular redox fluorometry has the potential to resolve the redox states of the various layers of the corneal epithelium. The posterior region of the epithelium is more active in mitochondrial respiration than the anterior region.
为检测兔角膜上皮细胞氧化还原状态的差异，对还原型吡啶核苷酸（PN）和氧化型黄素蛋白（Fp）的自发荧光进行了测量。将摘除眼球的兔眼置于装有麦卡里 - 考夫曼培养基的眼库眼容器中，使用眼氧化还原荧光法测量自发荧光，并将其作为深度的函数。PN信号分布均匀，而Fp信号在后上皮区域比在前上皮区域更强（p < 0.05）。作为组织氧化还原状态敏感指标的PN/Fp比值在后上皮区域较低。在培养基中加入1 mM氰化钾后，各层的该比值均显著增加（p < 0.001），前后区域之间的差异减小。这些结果表明，眼氧化还原荧光法有潜力分辨角膜上皮各层的氧化还原状态。上皮细胞的后区域在线粒体呼吸方面比前区域更活跃。
