Farstad I N, Halstensen T S, Fausa O, Brandtzaeg P
Laboratory for Immunohistochemistry and Immunopathology (LIIPAT), Institute of Pathology Rikshospitalet, Oslo, Norway.
Scand J Immunol. 1993 Nov;38(5):451-8. doi: 10.1111/j.1365-3083.1993.tb02587.x.
T-cell receptor gamma/delta+) (TcR gamma/delta+) lymphocytes in human Peyer's patches (PP) adjoining ileal mucosa were studied by monoclonal antibodies with paired immunofluorescence staining in situ and by flow-cytometric phenotyping of isolated cells. The proportion of gamma/delta+ T cells in the follicle-associated epithelium outside the M-cell areas (median 4.1%, range 2.2-30.1%) was similar to that in mucosal villous epithelium (median 4.4%, range 0.5-30.5%). Most intraepithelial gamma/delta cells (approximately 90%) expressed CD45R0 but only a few expressed CD8 (< 10%) and none L-selectin; a dominating subset (median 46%) employed the V delta 1/J delta 1 gene product (range 22-100%). The M-cell areas lacked gamma/delta cells but contained clustered CD20+ and CD3+ lymphocytes. The subepithelial PP dome area and interfollicular (T-cell) zones, as well as the mucosal lamina propria, contained very few gamma/delta cells (median 1.7%, range 0.4-8.9%) which were dominated (88-100%) by the V delta 2-encoded subset. Those in the dome area and lamina propria were often (approximately 75%) CD45R0+ (range 44-90%) while very few (approximately 2%) expressed L-selectin (range 0-15%). By contrast, CD45R0 expression on gamma/delta cells in the PP T-cell zones was relatively low (approximately 46%) and that of L-selectin relatively high (approximately 43%). In conclusion, TcR gamma/delta + cells are quite rare in human PP and belong mainly to the V delta 2-encoded subset, thus being different from most intraepithelial gamma/delta cells that probably have another origin. The L-selectin+ fraction of PP gamma/delta cells presumably represent newly recruited 'naive' T lymphocytes while CD45R0+ gamma/delta cells both in PP and lamina propria are probably antigen-primed.
通过单克隆抗体原位配对免疫荧光染色及分离细胞的流式细胞术表型分析，对毗邻回肠黏膜的人派尔集合淋巴结（PP）中的T细胞受体γ/δ +（TcRγ/δ +）淋巴细胞进行了研究。M细胞区域外的滤泡相关上皮中γ/δ + T细胞的比例（中位数4.1%，范围2.2 - 30.1%）与黏膜绒毛上皮中的比例（中位数4.4%，范围0.5 - 30.5%）相似。大多数上皮内γ/δ细胞（约90%）表达CD45R0，但只有少数表达CD8（<10%），且无L - 选择素表达；一个占主导地位的亚群（中位数46%）采用Vδ1/Jδ1基因产物（范围22 - 100%）。M细胞区域缺乏γ/δ细胞，但含有聚集的CD20 +和CD3 +淋巴细胞。上皮下PP圆顶区域和滤泡间（T细胞）区以及黏膜固有层中γ/δ细胞很少（中位数1.7%，范围0.4 - 8.9%），且以Vδ2编码的亚群为主（88 - 100%）。圆顶区域和固有层中的γ/δ细胞常（约75%）表达CD45R0（范围44 - 90%），而很少（约2%）表达L - 选择素（范围0 - 15%）。相比之下，PP T细胞区γ/δ细胞上CD45R0的表达相对较低（约46%），L - 选择素的表达相对较高（约43%）。总之，TcRγ/δ +细胞在人PP中相当罕见，主要属于Vδ2编码的亚群，因此与大多数可能有其他来源的上皮内γ/δ细胞不同。PPγ/δ细胞中L - 选择素阳性部分可能代表新招募的“未成熟”T淋巴细胞，而PP和固有层中的CD45R0 +γ/δ细胞可能已被抗原致敏。
