Ericsson O, Fridén M, Hellgren U, Gustafsson L L
Department of Clinical Pharmacology, Huddinge University Hospital, Sweden.
Ther Drug Monit. 1993 Aug;15(4):334-7. doi: 10.1097/00007691-199308000-00012.
The analysis of quinine in whole blood, plasma, urine, and samples dried on filter paper is described. Extraction was made with toluene followed by back-extraction into phosphate buffer. A reversed-phase liquid chromatography system with fluorescence detection was used. The within-day coefficient of variation of the method was 4-10% at the lower limit of determination (2 nM in plasma and 50 nM in whole blood, dried samples, and urine) and 2-4% at 10 microM. The quinine concentration was found to be lower in whole blood than in plasma (mean ratio, plasma-whole blood, 1.17). The concentration in capillary blood was lower than that in venous blood (mean ratio, capillary blood-venous blood, 0.93).
本文描述了全血、血浆、尿液以及滤纸上干燥样本中奎宁的分析方法。先用甲苯萃取，然后反萃取至磷酸盐缓冲液中。采用带荧光检测的反相液相色谱系统。该方法在测定下限（血浆中2 nM，全血、干燥样本和尿液中50 nM）时日内变异系数为4 - 10%，在10 μM时为2 - 4%。发现全血中奎宁浓度低于血浆（平均比值，血浆 - 全血，1.17）。毛细血管血中的浓度低于静脉血（平均比值，毛细血管血 - 静脉血，0.93）。
