Protas A F, Chaialo P P
Ukr Biokhim Zh (1978). 1993 Mar-Apr;65(2):73-8.
Superoxide dismutase (SOD) from rat brain cell nuclei has been distinguished. After chromatography on hydroxylapatite Sephacryl S200 and Mono S SOD can be purified 1200 times. It is Mn-depending enzyme, which has molecular weight 10 kDa and firmly associated with transcriptionally active chromatin. SOD is most active at high ion strength (1.2-2 M NaCl). The more sensitive method for SOD activity determination is suggested (modification of adrenaline autoxidation at pH 10.2).
已鉴别出来自大鼠脑细胞核的超氧化物歧化酶（SOD）。经羟磷灰石、Sephacryl S200和Mono S柱层析后，SOD可被纯化1200倍。它是一种依赖锰的酶，分子量为10 kDa，与转录活性染色质紧密相关。SOD在高离子强度（1.2 - 2 M NaCl）下活性最高。提出了一种更灵敏的SOD活性测定方法（在pH 10.2条件下肾上腺素自氧化法的改进）。
