类胡萝卜素被脂氧合酶共氧化：对亚油酸氢过氧化物形成的影响。

Co-oxydation of a carotenoid by the enzyme lipoxygenase: influence on the formation of linoleic acid hydroperoxides.

作者信息

Weber F, Grosch W

出版信息

Z Lebensm Unters Forsch. 1976;161(3):223-30. doi: 10.1007/BF01105807.

Abstract

A partially purified soybean lipoxygenase (L-3) was incubated for 15 min at pH 6.5 with linoleic acid and oxygen. Systems with and without the polyene glycoside crocin were compared. The system with crocin reacted with higher velocity with oxygen than did the control experiment without polyene. From the crocin 40% was destroyed. In the presence of crocin about 40% more linoleic acid hydroperoxides was formed than without the polyene but the linoleic acid break-down was equal in both experiments. L-3 peroxidises linoleic acid to 13L:13D:9L:9D-hydroperoxides in the proportions 43:11:21:25. In the presence of crocin the ratio of the isomers changed to 64:11:11:14.

摘要

将部分纯化的大豆脂氧合酶（L-3）在pH 6.5条件下与亚油酸和氧气一起孵育15分钟。比较了添加和不添加多烯糖苷藏红花素的体系。含有藏红花素的体系与氧气反应的速度比不添加多烯的对照实验更快。藏红花素中有40%被破坏。在有藏红花素存在的情况下，形成的亚油酸氢过氧化物比没有多烯时多约40%，但在两个实验中亚油酸的分解情况相同。L-3将亚油酸过氧化生成13L:13D:9L:9D-氢过氧化物，比例为43:11:21:25。在有藏红花素存在的情况下，异构体的比例变为64:11:11:14。