Kovàcs P, Bruch C, Fliedner T M
Acta Haematol. 1976;56(2):107-15. doi: 10.1159/000207926.
In soft agar cultures of canine blood leukocytes, an inhibition of colony formation was observed relative to the size of the inoculum. Analysis of the cellular composition of the inoculum suggested that this inhibition was associated with the number of polymorphonuclear leukocytes present. Removal of phagocytic cells by the iron ingestion method or selective destruction of granulocytes by freezing in the presence of dimethyl sulfoxide eliminated the inhibitory action on colony formation. In mixed cultures of canine bone marrow and antologous blood leukocytes, a similar inhibition of colony formation was observed. The results presented indicate that polymorphonuclear leukocytes, if present in a concentration exceeding 2.5X10(6)/ml of inoculum, inhibit in vitro granulocytic/monocytic colony formation.
在犬类血液白细胞的软琼脂培养中，观察到集落形成的抑制与接种物的大小有关。对接种物细胞组成的分析表明，这种抑制与存在的多形核白细胞数量有关。通过铁摄取法去除吞噬细胞或在二甲基亚砜存在下冷冻选择性破坏粒细胞消除了对集落形成的抑制作用。在犬类骨髓和自体血液白细胞的混合培养中，观察到类似的集落形成抑制。所呈现的结果表明，如果多形核白细胞的浓度超过每毫升接种物2.5×10⁶个，就会抑制体外粒细胞/单核细胞集落的形成。
