Calvo W, Fliedner T M, Steinbach I, Alcober V, Nothdurft W, Fache I
Am J Pathol. 1979 May;95(2):379-90.
The marrow matrix of total-body x-irradiated dogs (1200 R midline dose) was able to support effective hemopoiesis for several hundred days if the animals were transfused with their own mononuclear leukocytes collected from the blood prior to irradiation and preserved at ultralow temperatures. However, a lesion developed in the marrow, consisting of a fibrosis originating in conjunction with or from the endosteum. The fibrotic tissue substantially reduced the available marrow space in dogs with advanced lesions. The number of autologous, cryopreserved mononuclear leukocytes transfused ranged from 0.32 X 10(9) to 1.63 X 10(9)/kg body weight. The observation period extended to a maximum of 898 days after irradiation.
如果给全身接受过X射线照射（中线剂量1200拉德）的狗输注在照射前从其血液中采集并保存在超低温下的自身单核白细胞，那么这些狗的骨髓基质能够支持有效的造血功能达数百天。然而，骨髓中出现了一种病变，表现为由与骨内膜相关或起源于骨内膜的纤维化组成。在病变晚期的狗中，纤维化组织显著减少了可用的骨髓空间。输注的自体冷冻保存单核白细胞数量为每千克体重0.32×10⁹至1.63×10⁹个。观察期延长至照射后最长898天。
