Vadlamudi S, Hiremagalur B K, Tao L, Kalhan S C, Kalaria R N, Kaung H L, Patel M S
Department of Biochemistry, Case Western Reserve University School of Medicine, Cleveland, Ohio 44106.
Am J Physiol. 1993 Oct;265(4 Pt 1):E565-71. doi: 10.1152/ajpendo.1993.265.4.E565.
We have investigated pancreatic changes associated with hyperinsulinemia and an insulin secretory response to an oral glucose load in a new rat model for obesity. Male Sprague-Dawley pups were reared on a high-carbohydrate (HC) or high-fat (HF) formula by gastrostomy during the suckling period and were weaned onto a stock diet. These animals remained either nutritionally unchallenged or challenged with a high-sucrose diet during the postweaning period. The HC formula-fed animals showed increased insulin concentrations in the plasma and pancreas and also showed impaired insulin secretory response compared with mother-fed control or HF animals in adult life. Immunocytochemical and morphometric studies revealed that hyperinsulinemia in the HC animals during the preweaning period and also in adult life was associated with hypertrophy of beta-cells in the pancreas. The results show that consumption of a HC formula during the suckling period influences pancreatic islet morphology resulting in hyperinsulinemia which eventually leads to the development of obesity later in adult life.
我们在一种新的肥胖大鼠模型中，研究了与高胰岛素血症相关的胰腺变化以及对口服葡萄糖负荷的胰岛素分泌反应。雄性斯普拉格-道利幼崽在哺乳期通过胃造口术用高碳水化合物（HC）或高脂肪（HF）配方奶饲养，然后断奶并喂食常规饲料。这些动物在断奶后要么未受到营养挑战，要么接受高蔗糖饮食挑战。与成年后由母鼠喂养的对照动物或HF动物相比，用HC配方奶喂养的动物血浆和胰腺中的胰岛素浓度升高，并且胰岛素分泌反应受损。免疫细胞化学和形态计量学研究表明，断奶前和成年期HC动物的高胰岛素血症与胰腺β细胞肥大有关。结果表明，哺乳期食用HC配方奶会影响胰岛形态，导致高胰岛素血症，最终在成年后期引发肥胖。
