Laboratório de Imunobiotecnologia, Centro de Biotecnologia, Universidade Federal da Paraíba (UFPB), João Pessoa, Brazil.
Laboratório de Imunofarmacologia, Centro de Ciências da Saúde, Universidade Federal da Paraíba (UFPB), João Pessoa, Brazil.
Inflamm Res. 2017 Dec;66(12):1117-1130. doi: 10.1007/s00011-017-1092-9. Epub 2017 Sep 13.
Ouabain, an Na/K-ATPase inhibitor hormone, presents immunomodulatory actions, including anti-inflammatory effect on acute inflammation models.
In the present study, the effect of ouabain in a model of allergic airway inflammation induced by ovalbumin (OVA) was assessed.
Initially, it was observed that ouabain treatment inhibited cellular migration induced by OVA on bronchoalveolar lavage fluid (BALF), mostly granulocytes, without modulating macrophage migration. In addition, it was observed, by flow cytometry, that ouabain reduces CD3 lymphocytes cells on BALF. Furthermore, treatment with ouabain decreased IL-4 and IL-13 levels on BALF. Ouabain also promoted pulmonary histological alterations, including decreased cell migration into peribronchiolar and perivascular areas, and reduced mucus production in bronchioles regions observed through hematoxylin-eosin (HE) and by periodic acid-Schiff stain, respectively. Allergic airway inflammation is characterized by high OVA-specific IgE serum titer. This parameter was also reduced by the treatment with ouabain.
Therefore, our data demonstrate that ouabain negatively modulates allergic airway inflammation induced by OVA.
哇巴因是一种 Na/K-ATPase 抑制剂激素，具有免疫调节作用，包括对急性炎症模型的抗炎作用。
在本研究中，评估了哇巴因在卵清蛋白 (OVA) 诱导的过敏性气道炎症模型中的作用。
最初观察到哇巴因治疗抑制了 OVA 诱导的支气管肺泡灌洗液 (BALF) 中细胞迁移，主要是粒细胞，而不调节巨噬细胞迁移。此外，通过流式细胞术观察到哇巴因减少了 BALF 中的 CD3 淋巴细胞。此外，哇巴因治疗降低了 BALF 中的 IL-4 和 IL-13 水平。哇巴因还促进了肺组织学改变，包括减少细胞向细支气管周围和血管周围区域的迁移，以及通过苏木精-伊红 (HE) 和过碘酸-Schiff 染色分别观察到细支气管区域的粘液产生减少。过敏性气道炎症的特征是 OVA 特异性 IgE 血清滴度高。哇巴因治疗也降低了这一参数。
因此，我们的数据表明哇巴因负性调节 OVA 诱导的过敏性气道炎症。
