Pantano P, Kuhr W G
Department of Chemistry, University of California, Riverside 92521.
Anal Chem. 1993 Sep 15;65(18):2452-8. doi: 10.1021/ac00066a009.
Correlation of the chemical architecture of the surface of 10-microns-diameter carbon-fiber microelectrodes (illustrated by the fluorescence intensity of FITC-labeled carboxylates) and the rate of electron transfer of the surface (illustrated by the intensity of the electrogenerated chemiluminescence of luminol) allows the development of quantitative relationships between the chemical structure of an electrode surface and its electron-transfer properties. A fluorescence microscope equipped with a Peltier-cooled charge-coupled device was used to image these electrode surfaces with submicron spatial resolution. The total fluorescence emission observed at electrochemically treated electrodes was higher than that of controls while the voltammetric behavior and integrated ECL intensity of luminol were very similar. Imaging spectroscopy with submicron spatial resolution was able to demonstrate the microscopic heterogeneity of these surfaces and to assess the effect of the production of carboxylates on the rate of electron transfer of luminol.
10微米直径的碳纤维微电极表面的化学结构（由异硫氰酸荧光素标记的羧酸盐的荧光强度表示）与表面电子转移速率（由鲁米诺的电化学发光强度表示）之间的相关性，使得能够建立电极表面化学结构与其电子转移性质之间的定量关系。配备珀尔帖冷却电荷耦合器件的荧光显微镜用于以亚微米空间分辨率对这些电极表面进行成像。在电化学处理的电极上观察到的总荧光发射高于对照电极，而鲁米诺的伏安行为和积分电化学发光强度非常相似。具有亚微米空间分辨率的成像光谱能够证明这些表面的微观异质性，并评估羧酸盐的产生对鲁米诺电子转移速率的影响。
