Santarpia G, Venza M, Salmeri F M, Valenti A, Sottile A, Teti D
Institute of General Pathology, University of Messina, Italy.
Ann Clin Lab Sci. 1993 Sep-Oct;23(5):350-7.
Prostaglandin E2 (PGE2) appears to have an immunosuppressive role in human immunodeficiency virus (HIV) infection. Therefore, the effect was studied of PGE2 pretreatment of T lymphocytes from patients with lymphadenopathy associated syndrome (LAS) on the expression of CD25 and CD71 as well as plaque forming cell (PFC) generation in pokeweed mitogen (PWM)-driven cultures. The PGE2-treated or untreated T lymphocytes were cultured with B cells and monocytes in the presence of PWM. Both CD25 and CD71 expression were assessed with an immunofluorescence technique; PFC generation was tested by hemolysis. Before exposure to PWM, LAS lymphocytes showed activation as evidenced by high CD25 and CD71 expression and PFC generation. Pretreatment by PGE2 did not inhibit expression of activation markers and PFC generation in LAS cultures, in contrast to what happened in control cultures. Thus, LAS lymphocytes are activated in vivo and are less sensitive to PGE2 inhibition than normal lymphocytes.
前列腺素E2（PGE2）在人类免疫缺陷病毒（HIV）感染中似乎具有免疫抑制作用。因此，研究了用PGE2预处理淋巴结病相关综合征（LAS）患者的T淋巴细胞对在商陆有丝分裂原（PWM）驱动的培养物中CD25和CD71的表达以及空斑形成细胞（PFC）生成的影响。将经PGE2处理或未处理的T淋巴细胞与B细胞和单核细胞在PWM存在下进行培养。用免疫荧光技术评估CD25和CD71的表达；通过溶血试验检测PFC生成。在接触PWM之前，LAS淋巴细胞表现出活化，高CD25和CD71表达以及PFC生成证明了这一点。与对照培养物中发生的情况相反，PGE2预处理并未抑制LAS培养物中活化标志物的表达和PFC生成。因此，LAS淋巴细胞在体内被激活，并且比正常淋巴细胞对PGE2抑制的敏感性更低。
