Jezewska E, Sikora J, Słowik-Gabryelska A, Zeromski J
Department of Immunopathology, University Medical School, Poznań, Poland.
Arch Immunol Ther Exp (Warsz). 1993;41(1):51-6.
Lymphoid cells, isolated from malignant pleural effusions and collected from patients bearing primary lung carcinoma, were examined by means of indirect immunofluorescence and a panel of monoclonal antibodies vs several CD antigens. The percentages of CD4+ T lymphocytes were found to be significantly depressed in malignant effusions as compared to inflammatory ones. In relation to histological type of cancer it was especially evident in squamous cell and anaplastic carcinomas (small and large cell), in comparison to adenocarcinomas. Expression of T cell antigen receptor tau/delta (TCR-1) on T lymphocytes, demonstrated by BB3 MoAb (vs V delta 2), was significantly higher in malignant effusions as compared with non-malignant ones. This was not the case when A13 MoAb (equivalent of TCS 1) was used (vs V delta 1). Percentage values of NK cells, monocyte/granulocyte series activated cells and B lymphocytes did not differ significantly in malignant and non-malignant effusions. It is concluded that these are T lymphocyte subpopulations which are apparently distinct in both effusion groups examined.
从原发性肺癌患者的恶性胸腔积液中分离并收集淋巴细胞，通过间接免疫荧光法和一组针对几种CD抗原的单克隆抗体进行检测。结果发现，与炎性胸腔积液相比，恶性胸腔积液中CD4 + T淋巴细胞的百分比显著降低。就癌症的组织学类型而言，与腺癌相比，在鳞状细胞癌和间变性癌（小细胞和大细胞）中尤为明显。用BB3单克隆抗体（针对Vδ2）检测显示，T淋巴细胞上T细胞抗原受体tau/δ（TCR-1）的表达在恶性胸腔积液中明显高于非恶性胸腔积液。而使用A13单克隆抗体（相当于TCS 1，针对Vδ1）时情况并非如此。恶性和非恶性胸腔积液中NK细胞、单核细胞/粒细胞系列活化细胞和B淋巴细胞的百分比值没有显著差异。得出的结论是，在所检测的两个胸腔积液组中，这些T淋巴细胞亚群明显不同。
