Flurkey W H, Prentice D A, Fox M T, Hughes J P
Department of Chemistry, Indiana State University, Terre Haute 47809.
Biochem Biophys Res Commun. 1993 Oct 29;196(2):589-95. doi: 10.1006/bbrc.1993.2290.
Stathmin is a highly-conserved, cytosolic protein whose synthesis and phosphorylation is closely associated with growth and differentiation. Although conserved among vertebrates, stathmin has not been identified in plants. In the present study, anti-stathmin antibodies were generated against a synthetic peptide corresponding to amino-acid residues 32-44 of rat stathmin, and these antibodies were used to probe immunoblots of proteins from rat brain and mung bean. The antibodies recognized 12-kDa, 21-kDa and 22-kDa proteins in cytosolic fractions from mung bean leaves and a 12-kDa protein in cytosolic fractions from roots. The two larger proteins identified by the antibodies have apparent molecular weights and isoelectric points similar to those of rat brain stathmin. These results are the first to show that stathmin-like proteins are present in plants.
微管相关蛋白2是一种高度保守的胞质蛋白，其合成与磷酸化与生长和分化密切相关。尽管在脊椎动物中保守，但微管相关蛋白2尚未在植物中被鉴定出来。在本研究中，针对与大鼠微管相关蛋白2氨基酸残基32 - 44对应的合成肽产生了抗微管相关蛋白2抗体，并使用这些抗体探测大鼠脑和绿豆蛋白质的免疫印迹。这些抗体在绿豆叶片胞质部分识别出12 kDa、21 kDa和22 kDa的蛋白质，在根的胞质部分识别出12 kDa的蛋白质。抗体鉴定出的两种较大蛋白质的表观分子量和等电点与大鼠脑微管相关蛋白2相似。这些结果首次表明植物中存在类似微管相关蛋白2的蛋白质。
