Satta G, Grazi G, Varaldo P E, Fontana R
J Clin Pathol. 1979 Apr;32(4):391-5. doi: 10.1136/jcp.32.4.391.
A new test for the detection of bacterial phosphatase activity has been devised. The test is performed using agar media containing both methyl green (MG) and phenolphthalein diphosphate (PDP); in these media phosphatase-producing strains grow deep-green-stained colonies whereas non-producing strains do not. A total of 739 different strains were tested, including 593 staphylococci, 95 micrococci, 11 streptococci, 10 corynebacteria, 14 enterobacteria, and 16 candidae. All strains found phosphatase-positive according to the conventional phosphatase test displayed deep-green-stained colonies on MG-PDP media, whereas all phosphatase-negative strains showed unstained colonies on the same media. The main advantages of the present phosphatase test as compared with other conventional ones are that it is more simple to perform, it can reveal the phosphatase activity of colonies grown in deep agar, and can be incorporated into commercial multitest kits.
已设计出一种用于检测细菌磷酸酶活性的新测试方法。该测试使用含有甲基绿（MG）和酚酞二磷酸（PDP）的琼脂培养基进行；在这些培养基中，产生磷酸酶的菌株生长出深绿色染色的菌落，而不产生磷酸酶的菌株则不会。总共测试了739种不同的菌株，包括593株葡萄球菌、95株微球菌、11株链球菌、10株棒状杆菌、14株肠杆菌和16株念珠菌。根据传统磷酸酶测试发现的所有磷酸酶阳性菌株在MG-PDP培养基上均显示出深绿色染色的菌落，而所有磷酸酶阴性菌株在相同培养基上均显示出未染色的菌落。与其他传统测试相比，本磷酸酶测试的主要优点是操作更简单，可以揭示在深层琼脂中生长的菌落的磷酸酶活性，并且可以纳入商业多测试试剂盒中。
