Soro O, Grazi G, Varaldo P E, Satta G
Istituto di Microbiologia, Università degli Studi di Genova, Italy.
J Clin Microbiol. 1990 Dec;28(12):2707-10. doi: 10.1128/jcm.28.12.2707-2710.1990.
The phosphatase activities of colonies of staphylococcal strains belonging to the 10 species most frequently isolated from specimens of human origin were evaluated on media with undetermined inorganic phosphate contents and on media supplemented with known amounts of phosphates. All strains of all species tested were phosphatase positive on plates that were not supplemented with inorganic phosphates when the pH of the medium was high. In media supplemented with 0.3% phosphates at low and high pH, all strains of Staphylococcus aureus, almost all strains of S. epidermidis and S. xylosus, and none of the other species tested were phosphatase positive. Thirty S. simulans strains were grown in broths at pH 7 with and without phosphates, and the phosphatase activities of the cells were assayed at pH 5.5 and 8 in the absence of phosphates. At pH 8, all strains gave a strong positive reaction when grown in the absence of phosphates and a negative reaction when grown in the presence of these salts at 0.3%. It was concluded that all the species of staphylococci tested possess phosphatase activity and that the staphylococcal phosphatases are constitutive in some species and repressed in others.
对从人类标本中最常分离出的10种葡萄球菌菌株菌落的磷酸酶活性，在无机磷酸盐含量未确定的培养基上以及添加了已知量磷酸盐的培养基上进行了评估。当培养基pH值较高时，所有测试物种的所有菌株在未添加无机磷酸盐的平板上均呈磷酸酶阳性。在低pH和高pH下添加0.3%磷酸盐的培养基中，金黄色葡萄球菌的所有菌株、表皮葡萄球菌和木糖葡萄球菌的几乎所有菌株，以及其他测试物种的菌株均无磷酸酶阳性。30株模仿葡萄球菌菌株在有和无磷酸盐的情况下于pH 7的肉汤中培养，并在无磷酸盐的情况下于pH 5.5和8测定细胞的磷酸酶活性。在pH 8时，所有菌株在无磷酸盐条件下生长时产生强阳性反应，而在有0.3%这些盐的条件下生长时产生阴性反应。得出的结论是，所有测试的葡萄球菌物种都具有磷酸酶活性，并且葡萄球菌磷酸酶在某些物种中是组成型的，而在其他物种中受到抑制。
