Kwapinski G, Kwapinski E, Spurrier M
Health Lab Sci. 1976 Oct;13(4):262-6.
Soluble antigens were released by sonication from the cells of 60 primary cultures of Neiserria gonorrhoeae, 7 other species of neisseriae and 26 "mixed" cultures containing colonies of N. gonorrhoeae and other bacteria. Rabbits were injected with the soluble antigens of four strains of gonococci (clonal types 1 and 2), four other species of neisseriae and ten bacterial genera other than Neisseria. The antigens were tested against the non-absorbed antisera by a counter-immunoelectrophoresis method. It was found that the antiserum produced against the antigens of one N. gonorrhoeae strain, the strain GEK, reacted exclusively with the sonicates of all N. gonorrhoea cultures whether pure or mixed with other microorganisms. In contrast, the antisera to the antigens of all other N. gonorrhoeae strains cross-reacted with antigens of N. catarrhalis or N. sicca. Thus, the unique restricted reactivity of the anti-GEK antiserum permits its utilization as an immunological reagent for prompt identification of gonococcal cultures.
通过超声处理从60株淋病奈瑟菌原代培养物、7株其他奈瑟菌属菌株以及26株含有淋病奈瑟菌菌落和其他细菌的“混合”培养物的细胞中释放可溶性抗原。给兔子注射4株淋球菌（克隆型1和2）、4株其他奈瑟菌属菌株以及除奈瑟菌属之外的10个细菌属的可溶性抗原。采用对流免疫电泳法用未吸收的抗血清检测这些抗原。发现针对一株淋病奈瑟菌菌株GEK的抗原产生的抗血清仅与所有淋病奈瑟菌培养物的超声裂解物发生反应，无论这些培养物是纯培养还是与其他微生物混合培养。相比之下，针对所有其他淋病奈瑟菌菌株抗原的抗血清与卡他莫拉菌或干燥奈瑟菌的抗原发生交叉反应。因此，抗GEK抗血清独特的受限反应性使其可作为一种免疫试剂用于快速鉴定淋球菌培养物。
