Perkins W R, Minchey S R, Ahl P L, Janoff A S
Liposome Company, Inc., Princeton, New Jersey 08540.
Chem Phys Lipids. 1993 Sep;64(1-3):197-217. doi: 10.1016/0009-3084(93)90066-c.
Manipulating the process by which lipids assemble to form bilayer membranes has produced a myriad of protocol-dependent liposome types. For each of these systems the arrangement of bilayers is characteristic and can be described by parameters such as aqueous entrapment per mole lipid or captured volume, vesicle size distribution, the average number of lamellae per vesicle and shape. For specific applications as model systems or drug delivery systems specific characteristics are desired. Consequently over the years many techniques have evolved to better quantitate these parameters. Here we focus on and detail several methods to quantitate liposome captured volume. We also briefly describe the available methods to measure the other aforementioned physical properties and discuss their interdependency with captured volume.
操控脂质组装形成双层膜的过程已产生了无数种依赖于实验方案的脂质体类型。对于这些系统中的每一个，双层膜的排列都具有特征性，并且可以通过诸如每摩尔脂质的水包封量或捕获体积、囊泡大小分布、每个囊泡的平均层数和形状等参数来描述。对于作为模型系统或药物递送系统的特定应用，需要特定的特征。因此，多年来已经发展出许多技术来更好地定量这些参数。在这里，我们重点介绍并详细说明几种定量脂质体捕获体积的方法。我们还简要描述了测量其他上述物理性质的可用方法，并讨论了它们与捕获体积的相互依赖性。
