Grit M, Crommelin D J
Department of Pharmaceutics, Faculty of Pharmacy, University of Utrecht, The Netherlands.
Chem Phys Lipids. 1993 Sep;64(1-3):3-18. doi: 10.1016/0009-3084(93)90053-6.
In the first part of this article, chemical and physical stability of aqueous liposome dispersions have been addressed. Chemical stability of phospholipids has been considered in two parts: oxidation and hydrolysis. Major attention has been paid to hydrolysis kinetics of phospholipids as a function of pH, temperature, buffer concentration and ionic strength. Furthermore, the effect of chain length, head group, state of aggregation, addition of cholesterol and presence of charge on the hydrolysis kinetics of phospholipids has been dealt with. In the second part physical stability of chemically degraded liposome dispersions has been evaluated. In the final part quality control assays for liposome dispersions is presented and a HPLC method with a refractive index detector for the analysis of phospholipids from aqueous liposome dispersions is described.
在本文的第一部分，探讨了脂质体水性分散体的化学和物理稳定性。磷脂的化学稳定性分为两部分进行考虑：氧化和水解。主要关注了磷脂水解动力学与pH值、温度、缓冲液浓度和离子强度的关系。此外，还讨论了链长、头部基团、聚集状态、胆固醇的添加以及电荷的存在对磷脂水解动力学的影响。在第二部分中，评估了化学降解的脂质体分散体的物理稳定性。在最后一部分，介绍了脂质体分散体的质量控制检测方法，并描述了一种带有折光指数检测器的高效液相色谱法，用于分析脂质体水性分散体中的磷脂。
