Wen B Y
Shandong Institute of Industrial Hygiene and Occupational Disease, Ji'nan.
Zhonghua Yu Fang Yi Xue Za Zhi. 1993 May;27(3):151-3.
The average (OD values) and positive rates of specific IgE antibodies to a MDI-human serum albumin (HSA) conjugate in 25 workers exposed to MDI were significantly lower than those in 8 subjects of MDI asthma, while they were significantly higher than those in the 30 control subjects who had never bee exposed to MDI before. The positive rates of specific IgE antibodies in symptomatic MDI-workers were significantly higher than those in asymptomatic MDI-workers. The results of studies of specific IgE antigenic inhibition of serum ELISA activity confirmed the antigenic specificity to MDI-HSA. The average levels and positive rates of serum total IgE in 25 MDI-workers significantly increased when compared with 30 non-exposed subjects; while no significant difference when compared with 8 subjects of MDI asthma. Agreement rates of specific IgE were 85.7% with skin-prick test. The percentage of the acid alpha-naphthyl acetate esterase activity in T-cell of peripheral blood showed no statistically significant difference in all 3 groups. The results of the study suggested that the level of specific IgE was related to exposure to MDI and IgE mediated response played an important role in the allergic mechanism due to MDI, while it was not related to cell-immunological response.
25名接触二异氰酸甲苯酯（MDI）的工人中，针对MDI - 人血清白蛋白（HSA）偶联物的特异性IgE抗体的平均（光密度值）和阳性率显著低于8名MDI哮喘患者，而显著高于30名此前从未接触过MDI的对照受试者。有症状的MDI工人中特异性IgE抗体的阳性率显著高于无症状的MDI工人。血清ELISA活性的特异性IgE抗原抑制研究结果证实了对MDI - HSA的抗原特异性。与30名未接触者相比，25名MDI工人的血清总IgE平均水平和阳性率显著升高；与8名MDI哮喘患者相比则无显著差异。特异性IgE与皮肤点刺试验的符合率为85.7%。外周血T细胞中酸性α - 萘乙酸酯酶活性百分比在所有3组中均无统计学显著差异。研究结果表明，特异性IgE水平与MDI接触有关，IgE介导的反应在MDI所致过敏机制中起重要作用，而与细胞免疫反应无关。
