Zeiss C R, Kanellakes T M, Bellone J D, Levitz D, Pruzansky J J, Patterson R
J Allergy Clin Immunol. 1980 May;65(5):347-52. doi: 10.1016/0091-6749(80)90211-0.
Two workers are presented who were exposed to diphenylmethane diisocyanate (MDI) while coating pipes with a polyurethane foam. After a latent period of exposure, worker A developed immediate-onset asthma and worker B developed a clinical picture of hypersensitivity pneumonitis for which he was hospitalized. The antibody response of these workers to a conjugate of MDI with human serum albumin (MDI-HSA) was measured by gel precipitation, total antibody binding of 125I MDI-HSA, and specific IgG and IgE antibody by polystyrene-tube radioimmunoassay (PTRAI). Worker B had precipitating antibody to MDI-HSA by double immunodiffusion in gel. Both workers had high levels of IgG antibody specific for MDI-HSA which had some cross-reactivity with a conjugate or toluene diisocyanate and HSA. Total serum antibody binding of 125I MDI-HSA was 15 microgram/ml in worker A and 900 microgram/ml in worker B. Both workers had serum IgE antibody specific for MDI-HSA as measured by two PTRIA techniques. These results indicate that a marked immunologic response to MDI is possible in exposed workers and that hypersensitivity pneumonitis can occur subsequent to the inhalation of a low-molecular-weight chemical in the industrail setting.
本文介绍了两名在给管道涂抹聚氨酯泡沫时接触二苯基甲烷二异氰酸酯（MDI）的工人。在接触一段时间的潜伏期后，工人A出现速发型哮喘，工人B出现过敏性肺炎的临床表现并因此住院。通过凝胶沉淀法、¹²⁵I MDI-HSA的总抗体结合以及聚苯乙烯管放射免疫分析法（PTRAI）检测这些工人对MDI与人血清白蛋白偶联物（MDI-HSA）的抗体反应。工人B通过凝胶双向免疫扩散法检测出对MDI-HSA有沉淀抗体。两名工人针对MDI-HSA的IgG抗体水平都很高，其与甲苯二异氰酸酯和人血清白蛋白偶联物有一定交叉反应。工人A的¹²⁵I MDI-HSA血清总抗体结合量为15微克/毫升，工人B为900微克/毫升。通过两种PTRIA技术检测，两名工人血清中均有针对MDI-HSA的IgE抗体。这些结果表明，接触MDI的工人可能会出现明显的免疫反应，并且在工业环境中吸入低分子量化学物质后可能会发生过敏性肺炎。
