小鼠脾细胞膜免疫球蛋白重链：与人类和蟾蜍血清免疫球蛋白重链的比较。

Heavy chains of murine splenocyte membrane immunoglobulins: a comparison with the heavy chains of human and toad serum immunoglobulins.

作者信息

Warr G W, Marchalonis J J

出版信息

J Immunogenet. 1976 Aug;3(4):221-7. doi: 10.1111/j.1744-313x.1976.tb00578.x.

DOI:10.1111/j.1744-313x.1976.tb00578.x

Abstract

The mobilities of murine splenocyte surface immunoglobulin heavy chains were compared by polyacrylamide gel electrophoresis in SDS-containing buffers, with those of the heavy chains of human EgM, IgG, and IgD, and toad IgY. Human zota-chain showed a mobility only slightly faster than that of human mu-chain, and required double-labeling techniques for unequivocal resolution. The mobility of the slower murine splenocyte surface heavy chain was identical to that of human mu-chain, but that of the second splenocyte heavy chain was significantly faster than that of human zota-chain, and similar to that of the heavy chain of toad IgY. Although the second murine splenocyte heavy chain is clearly distinct from mu-chain, no physicochemical evidence exists which relates it directly to human zota-chain.

摘要

在含十二烷基硫酸钠（SDS）的缓冲液中，通过聚丙烯酰胺凝胶电泳比较了小鼠脾细胞表面免疫球蛋白重链与人IgM、IgG、IgD以及蟾蜍IgY重链的迁移率。人ζ链的迁移率仅比人μ链略快，并且需要双标记技术才能明确分辨。较慢的小鼠脾细胞表面重链的迁移率与人μ链相同，但第二条脾细胞重链的迁移率明显快于人的ζ链，与蟾蜍IgY重链的迁移率相似。尽管第二条小鼠脾细胞重链与μ链明显不同，但没有物理化学证据表明它与人ζ链有直接关系。