Goding J W, Warr G W, Warner N L
Proc Natl Acad Sci U S A. 1976 Apr;73(4):1305-9. doi: 10.1073/pnas.73.4.1305.
Lymphocyte surface antigens from spleen cells of several mouse strains were studied by cell surface radioiodination, extraction with detergent incubation with various antisera, and separation of complexes using protein A-containing staphylococci as a solid phase adsorbent. Complexes were then dissociated and analyzed by polyacrylamide gel electrophoresis in the presence of sodium diodecyl sulfate. Using this technique and an alloantiserum prepared in C57BL mice against CBA spleen cells, four distinct specific peaks of radioactivity were found with CBA spleen cells. These corresponded to H-2 and Ia antigens, immunoglobulin light chain, and a heavy chain previously proposed to be the murine homolog of the human delta chain. With the same serum, B10.BR spleen cells revealed only H-2 and Ia antigens, whereas C57BL.Ige (allotype congenic) spleen cells showed only the light chain and "delta" chain peaks. Depletion of immunoglobulin from the surface-iodinated preparations resulted in removal of the light chain and "delta" chain peaks. The tissue distribution and membrane expression of this "delta" chain antigen was then studied by indirect immunofluorescence with various C57BL derived alloantisera and lymphoid cells from C57.Ige allotype congenic mice. Significant numbers of positive cells were found in spleen, lymph nodes, and Peyer's patches, whereas few if any positive were found in bone marrow or thymus. No reaction was found between this molecule and alloantisera to any of the previously described immunoglobulin allotypes. It is proposed that these alloantisera to spleen cells recognise one allelic form of the murine "delta" chain coded for by a gene locus closely linked to the known structural genes for mouse immunoglobulin heavy chains. The designation Ig-5 is proposed for this new immunoglobulin heavy chain locus.
通过细胞表面放射性碘化、用去污剂提取、与各种抗血清孵育以及使用含蛋白A的葡萄球菌作为固相吸附剂分离复合物,对几种小鼠品系脾细胞的淋巴细胞表面抗原进行了研究。然后将复合物解离,并在十二烷基硫酸钠存在下通过聚丙烯酰胺凝胶电泳进行分析。使用该技术以及在C57BL小鼠中制备的针对CBA脾细胞的同种异体抗血清,在CBA脾细胞中发现了四个不同的放射性特异性峰。这些峰分别对应于H-2和Ia抗原、免疫球蛋白轻链以及先前提出的与人δ链同源的重链。使用相同的血清,B10.BR脾细胞仅显示H-2和Ia抗原,而C57BL.Ige(同种异型纯合子)脾细胞仅显示轻链和“δ”链峰。从表面碘化制剂中去除免疫球蛋白导致轻链和“δ”链峰消失。然后用来自C57.Ige同种异型纯合子小鼠的各种C57BL衍生的同种异体抗血清和淋巴细胞,通过间接免疫荧光研究这种“δ”链抗原的组织分布和膜表达。在脾脏、淋巴结和派伊尔结中发现了大量阳性细胞,而在骨髓或胸腺中几乎没有发现阳性细胞。未发现该分子与针对任何先前描述的免疫球蛋白同种异型的同种异体抗血清之间有反应。有人提出,这些针对脾细胞的同种异体抗血清识别由一个与小鼠免疫球蛋白重链已知结构基因紧密连锁的基因座编码的小鼠“δ”链的一种等位基因形式。建议将这个新的免疫球蛋白重链基因座命名为Ig-5。