酿酒酵母中肌动蛋白非互补（Anc）基因产物与肌动蛋白细胞骨架蛋白之间功能相互作用的遗传证据。
Genetic evidence for functional interactions between actin noncomplementing (Anc) gene products and actin cytoskeletal proteins in Saccharomyces cerevisiae.
作者信息
Vinh D B, Welch M D, Corsi A K, Wertman K F, Drubin D G
机构信息
Department of Molecular and Cell Biology, University of California, Berkeley 94720.
出版信息
Genetics. 1993 Oct;135(2):275-86. doi: 10.1093/genetics/135.2.275.
Abstract
We describe here genetic interactions between mutant alleles of Actin-NonComplementing (ANC) genes and actin (ACT1) or actin-binding protein (SAC6, ABP1, TPM1) genes. The anc mutations were found to exhibit allele-specific noncomplementing interactions with different act1 mutations. In addition, mutant alleles of four ANC genes (ANC1, ANC2, ANC3 and ANC4) were tested for interactions with null alleles of actin-binding protein genes. An anc1 mutant allele failed to complement null alleles of the SAC6 and TPM1 genes that encode yeast fimbrin and tropomyosin, respectively. Also, synthetic lethality between anc3 and sac6 mutations, and between anc4 and tpm1 mutations was observed. Taken together, the above results strongly suggest that the ANC gene products contribute to diverse aspects of actin function. Finally, we report the results of tests of two models previously proposed to explain extragenic noncomplementation.
摘要
我们在此描述肌动蛋白非互补（ANC）基因的突变等位基因与肌动蛋白（ACT1）或肌动蛋白结合蛋白（SAC6、ABP1、TPM1）基因之间的遗传相互作用。发现anc突变与不同的act1突变表现出等位基因特异性的非互补相互作用。此外，测试了四个ANC基因（ANC1、ANC2、ANC3和ANC4）的突变等位基因与肌动蛋白结合蛋白基因的无效等位基因之间的相互作用。一个anc1突变等位基因不能互补分别编码酵母丝束蛋白和原肌球蛋白的SAC6和TPM1基因的无效等位基因。此外，还观察到anc3和sac6突变之间以及anc4和tpm1突变之间的合成致死性。综上所述，上述结果强烈表明ANC基因产物在肌动蛋白功能的多个方面发挥作用。最后，我们报告了先前提出的用于解释基因外非互补的两个模型的测试结果。
相似文献
1
Genetic evidence for functional interactions between actin noncomplementing (Anc) gene products and actin cytoskeletal proteins in Saccharomyces cerevisiae.酿酒酵母中肌动蛋白非互补（Anc）基因产物与肌动蛋白细胞骨架蛋白之间功能相互作用的遗传证据。
Genetics. 1993 Oct;135(2):275-86. doi: 10.1093/genetics/135.2.275.
2
Screens for extragenic mutations that fail to complement act1 alleles identify genes that are important for actin function in Saccharomyces cerevisiae.筛选无法与act1等位基因互补的基因外突变，可鉴定出对酿酒酵母肌动蛋白功能重要的基因。
Genetics. 1993 Oct;135(2):265-74. doi: 10.1093/genetics/135.2.265.
3
Unexpected combinations of null mutations in genes encoding the actin cytoskeleton are lethal in yeast.编码肌动蛋白细胞骨架的基因中无效突变的意外组合在酵母中是致命的。
Mol Biol Cell. 1993 May;4(5):459-68. doi: 10.1091/mbc.4.5.459.
4
Mapping actin surfaces required for functional interactions in vivo.绘制体内功能相互作用所需的肌动蛋白表面图谱。
J Cell Biol. 1994 Jul;126(2):423-32. doi: 10.1083/jcb.126.2.423.
5
Suppressors of mdm20 in yeast identify new alleles of ACT1 and TPM1 predicted to enhance actin-tropomyosin interactions.酵母中mdm20的抑制因子鉴定出ACT1和TPM1的新等位基因，预计这些等位基因会增强肌动蛋白-原肌球蛋白的相互作用。
Genetics. 2000 Oct;156(2):523-34. doi: 10.1093/genetics/156.2.523.
6
Mutations that enhance the cap2 null mutant phenotype in Saccharomyces cerevisiae affect the actin cytoskeleton, morphogenesis and pattern of growth.在酿酒酵母中增强cap2缺失突变体表型的突变会影响肌动蛋白细胞骨架、形态发生和生长模式。
Genetics. 1993 Nov;135(3):693-709. doi: 10.1093/genetics/135.3.693.
7
The yeast gene, MDM20, is necessary for mitochondrial inheritance and organization of the actin cytoskeleton.酵母基因MDM20对于线粒体遗传和肌动蛋白细胞骨架的组织是必需的。
J Cell Biol. 1997 Apr 7;137(1):141-53. doi: 10.1083/jcb.137.1.141.
8
Dominant suppressors of yeast actin mutations that are reciprocally suppressed.酵母肌动蛋白突变的显性抑制因子，这些抑制因子相互抑制。
Genetics. 1989 Apr;121(4):675-83. doi: 10.1093/genetics/121.4.675.
9
end5, end6, and end7: mutations that cause actin delocalization and block the internalization step of endocytosis in Saccharomyces cerevisiae.end5、end6和end7：这些突变会导致肌动蛋白定位异常，并阻断酿酒酵母内吞作用的内化步骤。
Mol Biol Cell. 1995 Dec;6(12):1721-42. doi: 10.1091/mbc.6.12.1721.
10
Allele-specific suppression by formation of new protein-protein interactions in yeast.通过在酵母中形成新的蛋白质-蛋白质相互作用实现等位基因特异性抑制。
Genetics. 1997 Dec;147(4):1635-42. doi: 10.1093/genetics/147.4.1635.
引用本文的文献
1
A large-scale complex haploinsufficiency-based genetic interaction screen in Candida albicans: analysis of the RAM network during morphogenesis.在白念珠菌中进行大规模复杂的单倍体不足遗传互作筛选：形态发生过程中 RAM 网络的分析。
PLoS Genet. 2011 Apr;7(4):e1002058. doi: 10.1371/journal.pgen.1002058.
2
Anc1, a protein associated with multiple transcription complexes, is involved in postreplication repair pathway in S. cerevisiae.Anc1是一种与多种转录复合物相关的蛋白质，参与酿酒酵母的复制后修复途径。
PLoS One. 2008;3(11):e3717. doi: 10.1371/journal.pone.0003717. Epub 2008 Nov 13.
3
Modeling complex genetic interactions in a simple eukaryotic genome: actin displays a rich spectrum of complex haploinsufficiencies.在简单真核生物基因组中模拟复杂的基因相互作用：肌动蛋白呈现出丰富多样的复杂单倍剂量不足情况。
Genes Dev. 2007 Jan 15;21(2):148-59. doi: 10.1101/gad.1477507. Epub 2006 Dec 13.
4
Sometimes the result is not the answer: the truths and the lies that come from using the complementation test.有时结果并非答案：来自互补测验的真相与谎言。
Genetics. 2006 Sep;174(1):5-15. doi: 10.1534/genetics.106.064550.
5
The ctf13-30/CTF13 genomic haploinsufficiency modifier screen identifies the yeast chromatin remodeling complex RSC, which is required for the establishment of sister chromatid cohesion.ctf13-30/CTF13基因组单倍剂量不足修饰因子筛选鉴定出酵母染色质重塑复合体RSC，它是建立姐妹染色单体黏连所必需的。
Mol Cell Biol. 2004 Feb;24(3):1232-44. doi: 10.1128/MCB.24.3.1232-1244.2003.
6
exo1-Dependent mutator mutations: model system for studying functional interactions in mismatch repair.外切酶1依赖性诱变突变：用于研究错配修复中功能相互作用的模型系统
Mol Cell Biol. 2001 Aug;21(15):5142-55. doi: 10.1128/MCB.21.15.5142-5155.2001.
7
Rules of nonallelic noncomplementation at the synapse in Caenorhabditis elegans.秀丽隐杆线虫突触处非等位基因非互补规则。
Genetics. 2001 May;158(1):209-20. doi: 10.1093/genetics/158.1.209.
8
p150Glued, the largest subunit of the dynactin complex, is nonessential in Neurospora but required for nuclear distribution.动力蛋白激活蛋白复合体的最大亚基p150Glued在粗糙脉孢菌中并非必需，但对细胞核分布却是必需的。
Mol Biol Cell. 1996 May;7(5):731-42. doi: 10.1091/mbc.7.5.731.
9
Genetic interactions among cytoplasmic dynein, dynactin, and nuclear distribution mutants of Neurospora crassa.粗糙脉孢菌的细胞质动力蛋白、动力蛋白激活蛋白和核分布突变体之间的遗传相互作用。
Proc Natl Acad Sci U S A. 1996 May 14;93(10):4775-80. doi: 10.1073/pnas.93.10.4775.
10
Screens for extragenic mutations that fail to complement act1 alleles identify genes that are important for actin function in Saccharomyces cerevisiae.筛选无法与act1等位基因互补的基因外突变，可鉴定出对酿酒酵母肌动蛋白功能重要的基因。
Genetics. 1993 Oct;135(2):265-74. doi: 10.1093/genetics/135.2.265.
本文引用的文献
1
Screens for extragenic mutations that fail to complement act1 alleles identify genes that are important for actin function in Saccharomyces cerevisiae.筛选无法与act1等位基因互补的基因外突变，可鉴定出对酿酒酵母肌动蛋白功能重要的基因。
Genetics. 1993 Oct;135(2):265-74. doi: 10.1093/genetics/135.2.265.
2
Isolation and sequence of the gene for actin in Saccharomyces cerevisiae.酿酒酵母肌动蛋白基因的分离与测序。
Proc Natl Acad Sci U S A. 1980 Jul;77(7):3912-6. doi: 10.1073/pnas.77.7.3912.
3
A positive selection for mutants lacking orotidine-5'-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance.酵母中缺乏乳清苷-5'-磷酸脱羧酶活性的突变体的正向选择：5-氟乳清酸抗性。
Mol Gen Genet. 1984;197(2):345-6. doi: 10.1007/BF00330984.
4
Construction and genetic characterization of temperature-sensitive mutant alleles of the yeast actin gene.酵母肌动蛋白基因温度敏感突变等位基因的构建与遗传特征分析
Proc Natl Acad Sci U S A. 1984 Aug;81(15):4889-93. doi: 10.1073/pnas.81.15.4889.
5
Transformation of intact yeast cells treated with alkali cations.经碱金属阳离子处理的完整酵母细胞的转化
J Bacteriol. 1983 Jan;153(1):163-8. doi: 10.1128/jb.153.1.163-168.1983.
6
A ras-like protein is required for a post-Golgi event in yeast secretion.酵母分泌过程中高尔基体后事件需要一种类Ras蛋白。
Cell. 1987 May 22;49(4):527-38. doi: 10.1016/0092-8674(87)90455-7.
7
Yeast actin-binding proteins: evidence for a role in morphogenesis.酵母肌动蛋白结合蛋白：形态发生中作用的证据。
J Cell Biol. 1988 Dec;107(6 Pt 2):2551-61. doi: 10.1083/jcb.107.6.2551.
8
Identifying mutations in duplicated functions in Saccharomyces cerevisiae: recessive mutations in HMG-CoA reductase genes.鉴定酿酒酵母中重复功能的突变：HMG-CoA还原酶基因中的隐性突变
Genetics. 1987 Dec;117(4):645-55. doi: 10.1093/genetics/117.4.645.
9
High efficiency transformation of intact yeast cells using single stranded nucleic acids as a carrier.使用单链核酸作为载体对完整酵母细胞进行高效转化。
Curr Genet. 1989 Dec;16(5-6):339-46. doi: 10.1007/BF00340712.
10
Interacting genes identify interacting proteins involved in microtubule function in Drosophila.相互作用的基因鉴定出参与果蝇微管功能的相互作用蛋白。
Cell Motil Cytoskeleton. 1989;14(1):128-35. doi: 10.1002/cm.970140122.
Zotero 插件