酿酒酵母中肌动蛋白非互补（Anc）基因产物与肌动蛋白细胞骨架蛋白之间功能相互作用的遗传证据。
Genetic evidence for functional interactions between actin noncomplementing (Anc) gene products and actin cytoskeletal proteins in Saccharomyces cerevisiae.
作者信息
Vinh D B, Welch M D, Corsi A K, Wertman K F, Drubin D G
机构信息
Department of Molecular and Cell Biology, University of California, Berkeley 94720.
出版信息
Genetics. 1993 Oct;135(2):275-86. doi: 10.1093/genetics/135.2.275.
Abstract
We describe here genetic interactions between mutant alleles of Actin-NonComplementing (ANC) genes and actin (ACT1) or actin-binding protein (SAC6, ABP1, TPM1) genes. The anc mutations were found to exhibit allele-specific noncomplementing interactions with different act1 mutations. In addition, mutant alleles of four ANC genes (ANC1, ANC2, ANC3 and ANC4) were tested for interactions with null alleles of actin-binding protein genes. An anc1 mutant allele failed to complement null alleles of the SAC6 and TPM1 genes that encode yeast fimbrin and tropomyosin, respectively. Also, synthetic lethality between anc3 and sac6 mutations, and between anc4 and tpm1 mutations was observed. Taken together, the above results strongly suggest that the ANC gene products contribute to diverse aspects of actin function. Finally, we report the results of tests of two models previously proposed to explain extragenic noncomplementation.
摘要
我们在此描述肌动蛋白非互补（ANC）基因的突变等位基因与肌动蛋白（ACT1）或肌动蛋白结合蛋白（SAC6、ABP1、TPM1）基因之间的遗传相互作用。发现anc突变与不同的act1突变表现出等位基因特异性的非互补相互作用。此外，测试了四个ANC基因（ANC1、ANC2、ANC3和ANC4）的突变等位基因与肌动蛋白结合蛋白基因的无效等位基因之间的相互作用。一个anc1突变等位基因不能互补分别编码酵母丝束蛋白和原肌球蛋白的SAC6和TPM1基因的无效等位基因。此外，还观察到anc3和sac6突变之间以及anc4和tpm1突变之间的合成致死性。综上所述，上述结果强烈表明ANC基因产物在肌动蛋白功能的多个方面发挥作用。最后，我们报告了先前提出的用于解释基因外非互补的两个模型的测试结果。
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本文引用的文献
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Screens for extragenic mutations that fail to complement act1 alleles identify genes that are important for actin function in Saccharomyces cerevisiae.筛选无法与act1等位基因互补的基因外突变，可鉴定出对酿酒酵母肌动蛋白功能重要的基因。
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