4-Hyroxyaminoquinoline-1-oxide-induced ultrastructural changes in the guinea-pig exocrine pancreas.

4-hydroxyaminoquinoline, a carcinogen was injected into guinea-pigs intravenously in a single dose of 22-5 mg/kg. The sequential morphological changes in the exocrine cells of the pancreas were studied by electron microscopy at selected intervals between 18 and 60 hr. The earliest alterations in the acinar cells comprised nucleolar segregation into granular and fibrillar components and minimal dilatation of endoplasmic reticulum. At later intervals all the cytoplasmic organelles appeared altered. The endoplasmic reticular channels were markedly dilated with depletion of membrane bound and free ribosomes. The mitochondria were swollen and irregular with disruption of limiting membranes and cristae. The zymogen granules were markedly reduced or completely absent. The hyaloplasm contained morphologically variable cytoplasmic degenerative bodies. In addition to the acinar cells, centriacinar and ductal epithelial cells showed similar changes.