Gilhar A, Etzioni A, Pillar T, Eidelman S
Skin Research Laboratory, Rappaport School of Medicine, Technion, Haifa, Israel.
Isr J Med Sci. 1993 Oct;29(10):609-12.
Increased expression of class II antigens (HLA-DR, human:Ia murine) on epithelial cells (EC) such as keratinocytes and gut EC suggests a role of the epithelium in the inflammatory response. It has been shown that injection of normal mouse serum (NMS) into nude mice caused an induction of Ia antigen by keratinocytes of the nude mice. The aim of the present study was to determine whether such induction could be observed in gut EC and whether it can be inhibited by cyclosporine A (CyA). Forty nude mice were divided into 4 groups of 10 each, designated A-D. Group A was injected with 0.1 ml of NMS; groups B and C were also injected with NMS and treated with oral CyA for 10 days. The dosage in group B was 30 g/ml and in group C 60 g/ml of drinking water. Group D was injected with serum of nude mice and served as a control group. Biopsies of small intestine and colon were obtained from each mouse and analyzed by indirect immunoperoxidase to identify Ia expression. Small intestine and colon EC were induced to express Ia antigen in most of the mice in groups A and B. A striking reduction in Ia expression was noted in group C. The differences between groups A and C concerning Ia expression on small intestine and colon EC were statistically significant. Our results demonstrate that the nude mouse may serve as a model for the study of Ia expression on gut EC, and that CyA can suppress the expression of Ia antigen also in the gut.
角质形成细胞和肠道上皮细胞等上皮细胞（EC）上II类抗原（人类为HLA - DR，鼠类为Ia）表达增加，提示上皮细胞在炎症反应中发挥作用。已有研究表明，向裸鼠注射正常小鼠血清（NMS）可导致裸鼠角质形成细胞诱导Ia抗原表达。本研究的目的是确定在肠道上皮细胞中是否能观察到这种诱导作用，以及它是否能被环孢素A（CyA）抑制。40只裸鼠被分为4组，每组10只，分别标记为A - D组。A组注射0.1 ml NMS；B组和C组也注射NMS，并口服CyA治疗10天。B组饮用水中CyA剂量为30 μg/ml，C组为60 μg/ml。D组注射裸鼠血清作为对照组。从每只小鼠获取小肠和结肠活检组织，通过间接免疫过氧化物酶法分析以鉴定Ia表达。A组和B组的大多数小鼠中小肠和结肠上皮细胞被诱导表达Ia抗原。C组Ia表达显著降低。A组和C组在小肠和结肠上皮细胞Ia表达方面的差异具有统计学意义。我们的结果表明，裸鼠可作为研究肠道上皮细胞Ia表达的模型，并且CyA也能抑制肠道中Ia抗原的表达。
