Zhou F X, Krull I S, Feibush B
Department of Chemistry, Northeastern University, Boston, MA 02115.
J Chromatogr. 1993 Sep 8;619(1):93-101. doi: 10.1016/0378-4347(93)80450-i.
A simple, highly sensitive and selective method is described for adamantanamine determination in plasma and urine by high-performance liquid chromatography with fluorescence detection. The method involved a simultaneous extraction and derivatization of biological fluids with a 9-fluoreneacetate (9-FA) solid-phase derivatization reagent. This approach eliminated tedious sample preparation steps and provided automatic derivatization with selective and efficient sample clean-up for direct injection of biological fluids. Derivatized adamantanamine was separated under conventional reversed-phase conditions and determined by fluorescence detection. The optimization and validation of the derivatization method with the 9-FA solid-phase reagent is described.
描述了一种简单、高灵敏且具选择性的方法，用于通过高效液相色谱-荧光检测法测定血浆和尿液中的金刚烷胺。该方法采用9-芴乙酸酯（9-FA）固相衍生化试剂对生物样品进行同时萃取和衍生化。此方法省去了繁琐的样品制备步骤，提供了自动衍生化以及选择性高效的样品净化，可直接进样生物样品。衍生化后的金刚烷胺在常规反相条件下进行分离，并通过荧光检测进行测定。文中还描述了使用9-FA固相试剂的衍生化方法的优化和验证。
