Szulc M, Krull I S
Northeastern University, Department of Chemistry, Boston, MA 02115.
Biomed Chromatogr. 1994 Sep-Oct;8(5):212-8. doi: 10.1002/bmc.1130080504.
A polymer immobilized o-nitrobenzophenone reagent was prepared for analysis of amine drugs in micellar electrokinetic chromatography (MEKC). A model compound, propylamine, was used to characterize the reagent's performance in MEKC. Derivatizations were performed on the CE instrument with reagent in the sample vial. The yielded derivative was directly sampled from the reaction mixture, and directly injected onto the MEKC system. The derivatization reagent was also applied to the derivatization of n-alkyl amine mixtures and amino acids. The method was validated for adamantanamine in urine and in plasma by single-blind spike analysis. Precisions and accuracies for all samples were less than 6.0% for urine samples and 10% for plasma samples. The procedure was a direct injection technique requiring minimal sample preparation for the analysis of drugs in biofluids.
制备了一种聚合物固定化的邻硝基二苯甲酮试剂，用于在胶束电动色谱法（MEKC）中分析胺类药物。使用模型化合物丙胺来表征该试剂在MEKC中的性能。在CE仪器上，于样品瓶中用试剂进行衍生化反应。生成的衍生物直接从反应混合物中取样，并直接注入到MEKC系统中。该衍生化试剂还应用于正烷基胺混合物和氨基酸的衍生化。通过单盲加标分析，验证了该方法用于尿液和血浆中金刚烷胺的检测。所有样品的精密度和准确度，尿液样品小于6.0%，血浆样品小于10%。该方法是一种直接进样技术，分析生物流体中的药物时所需样品前处理极少。
