Cowan R G, Winnie G B
Department of Pediatrics, Albany Medical College, New York 12208.
J Clin Immunol. 1993 Sep;13(5):359-70. doi: 10.1007/BF00920245.
To explore possible mechanisms for the association between elevated immunoglobulin levels and lower pulmonary function in cystic fibrosis patients, we measured serum IgG subclass levels and anti-P. aeruginosa IgG subclass titers and correlated levels with neutrophil phagocytosis and chemotaxis. Serum was obtained from 13 cystic fibrosis patients colonized with the same serotype of P. aeruginosa, 12 noncolonized patients, and 12 normal volunteers. All anti-P. aeruginosa IgG subclass titers were elevated in serum from colonized patients. IgG3 level and anti-P. aeruginosa IgG3 titer were inversely correlated with pulmonary function. Phagocytosis of P. aeruginosa by neutrophils correlated with serum IgG3 level and was increased by opsonization with serum from colonized patients. Chemotactic index was increased in serum from colonized patients and inversely correlated with pulmonary function chest roentgenogram score. Chemotactic index directly correlated with anti-P. aeruginosa IgG3 titer and serum IgG3. These data demonstrate that cystic fibrosis patients with increased IgG3 levels are in poorer clinical condition and that their serum enhances neutrophil function. Such patients may have increased pulmonary inflammation with subsequent lung damage.
为探究囊性纤维化患者免疫球蛋白水平升高与肺功能降低之间关联的潜在机制，我们检测了血清IgG亚类水平及抗铜绿假单胞菌IgG亚类滴度，并将这些水平与中性粒细胞吞噬作用和趋化性进行关联分析。血清取自13例感染相同血清型铜绿假单胞菌的囊性纤维化患者、12例未感染患者以及12名正常志愿者。所有感染患者血清中的抗铜绿假单胞菌IgG亚类滴度均升高。IgG3水平及抗铜绿假单胞菌IgG3滴度与肺功能呈负相关。中性粒细胞对铜绿假单胞菌的吞噬作用与血清IgG3水平相关，且经感染患者血清调理后增强。感染患者血清的趋化指数升高，且与肺功能胸部X线评分呈负相关。趋化指数与抗铜绿假单胞菌IgG3滴度及血清IgG3直接相关。这些数据表明，IgG3水平升高的囊性纤维化患者临床状况较差，且其血清可增强中性粒细胞功能。此类患者可能肺部炎症增加，继而导致肺损伤。
